The role of aspartic acid in reducing coral calcification under ocean acidification conditions
The role of aspartic acid in reducing coral calcification under ocean acidification conditions
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DOI：
10.1038/s41598-020-69556-0
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发表时间：
2020-07-30
影响因子：
4.6
通讯作者：
Allison, Nicola
中科院分区：
文献类型：
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作者：
Kellock, Celeste;Cole, Catherine;Allison, Nicola
生物分子在调节CaCO 3生物矿物的沉淀中起着关键作用，但对它们对海洋酸化的反应知之甚少。我们分析了大量的，热带滨珊瑚属的骨骼晶内氨基酸。在不同海水pCO下培养的珊瑚（2）。我们发现，总氨基酸、天冬氨酸/天冬酰胺（Asx）、谷氨酸/谷氨酰胺和丙氨酸的浓度与海水pCO（2）呈正相关，与海水pH呈负相关。几乎所有珊瑚钙化率的差异都可以用骨骼总氨基酸、Asx、丝氨酸和丙氨酸浓度与钙化介质pH值（可用于支持钙化的溶解无机碳的可能指标）相结合。我们发现，天冬氨酸抑制文石沉淀从海水中在体外，在pH值，饱和状态和近似的天冬氨酸浓度推断发生在珊瑚钙化网站。降低海水饱和状态和增加[天冬氨酸]，如在高pCO（2）下发生在一些珊瑚中，都有助于增加抑制程度，表明生物分子可能有助于降低海洋酸化下的珊瑚钙化率。
Biomolecules play key roles in regulating the precipitation of CaCO3 biominerals but their response to ocean acidification is poorly understood. We analysed the skeletal intracrystalline amino acids of massive, tropical Porites spp. corals cultured over different seawater pCO(2). We find that concentrations of total amino acids, aspartic acid/asparagine (Asx), glutamic acid/glutamine and alanine are positively correlated with seawater pCO(2) and inversely correlated with seawater pH. Almost all variance in calcification rates between corals can be explained by changes in the skeletal total amino acid, Asx, serine and alanine concentrations combined with the calcification media pH (a likely indicator of the dissolved inorganic carbon available to support calcification). We show that aspartic acid inhibits aragonite precipitation from seawater in vitro, at the pH, saturation state and approximate aspartic acid concentrations inferred to occur at the coral calcification site. Reducing seawater saturation state and increasing [aspartic acid], as occurs in some corals at high pCO(2), both serve to increase the degree of inhibition, indicating that biomolecules may contribute to reduced coral calcification rates under ocean acidification.