LONG-TERM INVITRO CULTIVATION OF PLASMODIUM-BERGHEI
LONG-TERM INVITRO CULTIVATION OF PLASMODIUM-BERGHEI
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DOI：
10.1016/0020-7519(87)90099-3
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发表时间：
1987-10-01
影响因子：
4
通讯作者：
RENAPURKAR, DM
中科院分区：
文献类型：
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作者：
RAMAIYA, ML;KAMATH, VR;RENAPURKAR, DM
使用Trager和詹森（1976）的培养皿蜡烛罐法建立伯氏疟原虫的长期体外培养。在22、27和37 ℃下建立培养物。C.在27 ℃下观察到最佳生长。C，随后的培养在该温度下进行。RPMI 1640培养基通过在培养基中加入额外的葡萄糖（1 mg ml-1）和细菌蛋白胨（1 mg ml-1）来改良。发现该培养基适合于体外维持小鼠红细胞。使用蜡烛罐和这种改良的RPMI 1640培养基将伯氏疟原虫培养物维持45周。
Long-term in vitro culture of Plasmodium berghei was established using the Petri dish candle jar method of Trager and Jensen (1976). Cultures were established at 22, 27 and 37.degree. C. As optimal growth was observed at 27.degree. C, subsequent cultivation was carried out at this temperature. RPMI 1640 medium was modified by incorporating additional glucose (1 mg ml-1) and bactopeptone (1 mg ml-1) in the medium. This medium was found suitable for maintenance of mouse erythrocytes in vitro. P. berghei cultures were maintained using candle jars and this modified RPMI 1640 medium for 45 weeks.