Effect of nitrite on growth and microcystins production of Microcystis aeruginosa PCC7806
Effect of nitrite on growth and microcystins production of Microcystis aeruginosa PCC7806
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DOI：
10.1007/s10811-010-9558-y
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发表时间：
2011-08-01
影响因子：
3.3
通讯作者：
Dai, Shugui
中科院分区：
文献类型：
--
作者：
Chen, Weimin;Liu, Hao;Dai, Shugui
作为一种常见的污染物，亚硝酸盐在某些水生系统中的浓度可接近15 mg NO（2）（-）-N L（-1）。铜绿微囊藻水华在富营养化水体中普遍存在。在本研究中，M.铜绿假单胞菌暴露于0 ~ 15 mg NO（2）（-）-N L（-1）的亚硝酸盐浓度下，其对亚硝酸盐的反应为：铜绿假单胞菌进行了研究。随着亚硝酸盐浓度的增加，比生长速率、最大细胞密度、光饱和光合速率（P（m）（chla））、暗呼吸速率（R（d）（chla））和表观光合效率（alpha（chla））均显著下降。电导率和丙二醛含量的调查显示，细胞膜损伤和明显的泄漏，在高亚硝酸盐水平的条件下，由于氧化应激增强的细胞内内容物。细胞内微囊藻毒素（MC）-LR含量在10 mg NO（2）（-）-N L（-1）时达到最高值，而细胞外MC-LR含量在15 mg NO（2）（-）-N L（-1）时持续增加，这是由于细胞内物质渗漏增加所致。说明高浓度亚硝酸盐对M.铜绿假单胞菌的生长是通过增加氧化应激，破坏细胞膜和减少光合作用来实现的。而亚硝酸盐浓度的适度增加则促进了毒素的产生和释放。
As a common pollutant, nitrite concentrations can approach 15 mg NO (2) (-) -N L(-1) in some aquatic systems. Microcystis aeruginosa blooms are common and widespread in eutrophic freshwater bodies. In this study, M. aeruginosa was exposed to nitrite concentrations ranging from 0 to 15 mg NO (2) (-) -N L(-1), and the responses of M. aeruginosa were investigated. The specific growth rates, maximum cell densities, light-saturated photosynthetic rates (P(m) (chla) ), dark respiration rates (R(d) (chla) ), and apparent photosynthetic efficiencies (alpha(chla) ) showed a significant decline with nitrite concentrations increasing. Electrical conductivity and malondialdehyde contents investigation revealed cell membrane damage and apparent leakage of intracellular contents under high nitrite level conditions due to oxidative stress enhancement. Intracellular microcystin (MC)-LR content reached the highest value at 10 mg NO (2) (-) -N L(-1); however, extracellular MC-LR contents showed a continuous increase until 15 mg NO (2) (-) -N L(-1) owing to the increasing leakage of intracellular contents. These results elucidated that the high-level nitrite inhibited M. aeruginosa growth by rising oxidative stress, damaging cell membrane, and reducing photosynthesis. However, the moderate increase in nitrite concentrations promoted toxin production and release of toxin.