Dilute povidone-iodine solutions inhibit human skin fibroblast growth
Dilute povidone-iodine solutions inhibit human skin fibroblast growth
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DOI：
10.1046/j.1524-4725.2002.01161.x
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发表时间：
2002-03-01
影响因子：
2.4
通讯作者：
Pratt, L
中科院分区：
文献类型：
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作者：
Balin, AK;Pratt, L
背景聚维酮碘溶液是广泛使用的高效防腐剂。虽然通常以全强度使用，但该浓度似乎对参与伤口愈合的细胞有毒。很少有关于聚维酮碘毒性的系统研究报告。使用体外细胞培养评估10%聚维酮碘溶液的各种稀释度对人二倍体成纤维细胞生长的影响。本研究的目的是使用体外模型系统地评价聚维酮碘对活细胞的毒性。将成人皮肤成纤维细胞和胎儿肺成纤维细胞以3000- 10，000个细胞/cm 2的不同接种密度传代培养，并在含有5%O-2、5%CO2和90%N-2的气氛下在聚苯乙烯组织培养瓶中生长。细胞在含有不同浓度聚维酮碘（1%、0.1%、0.025%、0.01%和0%）的培养基中生长。在我们最初的研究中，细胞附着减少了0.1%和1%聚维酮碘;随后的实验通过在接种后24小时改变培养基以含有聚维酮碘来进行。通过每48小时计数一式三份培养物，持续250-300小时，绘制生长曲线。成纤维细胞生长在0.01%和0.025%时逐渐减慢，并且被0.1%和1%聚维酮碘溶液完全抑制。在有限暴露于聚维酮碘稀释液后，细胞生长部分恢复。这项研究表明，即使是稀释的聚维酮碘溶液对人成纤维细胞也是有毒的。结果表明，将聚维酮碘放置在开放性伤口上时应谨慎，并应避免与未污染的活体组织长时间接触。
BACKGROUND. Povidone-iodine solutions are widely used and highly effective antiseptics. Although commonly used at full strength, this concentration appears to be toxic to the cells involved in wound healing. Few systematic studies of povidone-iodine toxicity have been reported. The effects of various dilutions of 10% povidone-iodine solution on the growth of human diploid fibroblasts were assessed using in vitro cell culture.OBJECTIVE. The purpose of this study was to systematically evaluate the toxicity of povidone-iodine on living cells using an in vitro model.METHODS. Adult skin fibroblasts and fetal lung fibroblasts were subcultivated at various seeding densities of 3000-10,000 cells/cm(2) and grown in polystyrene tissue culture flasks under an atmosphere containing 5% O-2, 5% CO2, and 90% N-2. Cells were grown in a medium containing various concentrations of povidone-iodine (1%, 0.1%, 0.025%, 0.01%, and 0%). Cell attachment was reduced by 0.1% and 1% povidone-iodine in our initial studies; subsequent experiments were performed by changing the medium to contain the povidonc-iodine 24 hours after seeding. Growth curves were performed by counting triplicate cultures every 48 hours for 250-300 hours.RESULTS. Fibroblast growth was progressively retarded at 0.01% and 0.025%, and totally inhibited by 0.1% and 1% povidone-iodine solutions. Partial recovery of cell growth after limited exposure of cultures to dilute solutions of povidone-iodine was noted.CONCLUSION. This study shows that even dilute solutions of povidone-iodine arc toxic to human fibroblasts. The results indicate that caution should be used when povidone-iodine is placed on an open wound, and that prolonged contact with viable uncontaminated tissue should be avoided.