In vivo biometry in the mouse eye with low coherence interferometry
In vivo biometry in the mouse eye with low coherence interferometry
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DOI：
10.1016/j.visres.2004.05.018
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发表时间：
2004-01-01
期刊：
影响因子：
1.8
通讯作者：
Schaeffel, F
中科院分区：
文献类型：
--
作者：
Schmucker, C;Schaeffel, F
目的.近视小鼠模型的主要缺点是眼睛尺寸不能在体内测量，并且死后组织学技术的分辨率有限。我们已经测试了一种新开发的技术，光学低相干干涉法（OLCI），适用于短距离测量的Meditec，卡尔蔡司，耶拿，德国（“ACMaster”）的潜力。使用这种技术，在具有正常视力的小鼠和剥夺形觉后进行眼生物测量。在23只25-53日龄的小鼠中测量眼轴长度、角膜厚度和前房深度，并在不同年龄组中确定相同眼睛中重复测量的标准差以及个体内和个体间变异性。将数据与先前研究的数据进行比较，其中生物统计学数据来自冷冻切片[Vision Res. 44（2004）1857]。通过自动红外摄影验光测量屈光度。小鼠在正常视觉暴露或单眼剥夺形式视觉14天。使用OLCI，可以确定眼轴长度，平均标准差为8.0 +/- 2.9 μ m，角膜厚度为3.5 +/- 2.1 μ m，前房深度为10.6 +/- 12.3 μ m。在具有正常视觉体验的个体小鼠的左眼和右眼中，眼轴长度、角膜厚度和前房深度均无显著差异（眼轴长度之间的平均绝对差异：17 +/- 18 μ m，角膜厚度之间的平均绝对差异：5.1 +/- 4.8 μ m，前房深度之间的平均绝对差异：16.7 +/- 14.8 μ m）。与先前在冷冻切片中发现的变异性相比，OLCI的眼轴长度测量的变异性是2.7倍less. After两周的形觉剥夺，OLCI显示，与对侧同伴眼相比，闭塞眼的眼轴显著延长（+38 +/-36妈妈或1.16%，p = 0.045，n = 7，配对t检验）。在该样品中，在闭合的眼睛中没有观察到伴随的近视移位，但是考虑到小鼠眼睛生长对视觉剥夺的固有可变响应，该观察结果并不意外。OLCI在活体小鼠中具有足够的分辨率，可检测体内眼轴长度变化，相当于2 D的屈光变化。使用该技术，证实了小鼠眼睛通过轴向伸长响应形觉剥夺，类似于其他动物模型的眼睛。尽管轴向伸长，但该样本中缺乏近视移位，这表明当使用小鼠眼睛作为研究近视的模型时，生物识别数据特别重要。(C)2004爱思唯尔有限公司保留所有权利。
Purpose. A major drawback of the mouse model of myopia is that the ocular dimensions cannot be measured in vivo, and that histological techniques post-mortem suffer from limited resolution. We have tested the potential of a newly developed technique, optical low coherence interferometry (OLCI), adapted for short measurement distances by Meditec, Carl Zeiss, Jena, Germany (the "ACMaster"). Using this technique, ocular biometry was performed in mice with normal vision and after deprivation of form vision.Methods. Axial eye length, corneal thickness and anterior chamber depth were measured in 23 mice, aged 25-53 days, and standard deviations from repeated measurements in the same eyes, as well as intra-individual and inter-individual variability were determined in different age groups. The data were compared to those from a preceding, study in which biometrical data were obtained from frozen sections [Vision Res. 44 (2004) 1857]. Refractions were measured by automated infrared photorefraction. Mice had either normal visual exposure or were monocularly deprived of form vision for 14 days.Results. Using OLCI, axial length could be determined with an average standard deviation of 8.0 +/- 2.9 mum, corneal thickness with 3.5 +/- 2.1 mum, and anterior chamber depth with 10.6 +/- 12.3 mum. Neither axial length, nor corneal thickness, nor anterior chamber depth were significantly different in left and right eyes of individual mice that had normal visual experience (mean absolute difference between axial lengths: 17 +/- 18 mum, between corneal thickness 5.1 +/- 4.8 mum, and between anterior chamber depths 16.7 +/- 14.8 mum). Compared to the variability that was previously found in frozen sections, the variability of axial length measurements with OLCI was 2.7 times less. After two weeks of form deprivation, OLCI revealed a significant axial elongation in the occluded eyes, compared to the contralateral fellow eyes (+38 +/- 36 mum or 1.16%, p = 0.045, n = 7, paired t-test). In this sample, no accompanying myopic shift was observed in the Occluded eyes but this observation is not unexpected given the inherently variable responses of mouse eye growth to visual deprivation.Conclusion. OLCI had sufficient resolution in living mice to detect axial length changes in vivo that were equivalent to a dioptric change of 2 D. Using this technique, it was confirmed that mouse eyes respond to form deprivation by axial elongation, similar to the eyes of other animal models. The lack of a myopic shift in this sample, despite the axial elongation, demonstrates that biometric data are particularly important when the mouse eye is used as a model to Study myopia. (C) 2004 Elsevier Ltd. All rights reserved.