A minimum information standard for reproducing bench-scale bacterial cell growth and productivity
A minimum information standard for reproducing bench-scale bacterial cell growth and productivity
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DOI：
10.1038/s42003-018-0220-6
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发表时间：
2018-01-01
影响因子：
5.9
通讯作者：
Salit, Marc
中科院分区：
文献类型：
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作者：
Hecht, Ariel;Filliben, James;Salit, Marc
复制、交换、比较和借鉴彼此的工作成果是技术进步的基础。推进生物技术需要可靠地再利用工程生物。工程生物的可靠再利用需要可再生的生长和生产力。在这里，我们确定了对我们的工程生物体的生长和生产力影响最大的实验因素，以证明生物技术的可重复性。我们提出了一个草案的最低信息标准的工程生物实验（MIEO）的基础上，这种方法。本研究考察了22个因素对大肠杆菌工程菌生产番茄红素的影响，18个因素对大肠杆菌工程菌生产番茄红素的影响。大肠杆菌改造产生红色荧光蛋白。容器几何形状和振摇对产品滴度和产量的影响最大。我们在两种不同的重现性条件下重现了我们的结果：使用条件（不同的部分析因实验）和时间（在4个月内的12个不同的日子进行了48次生物重复）。
Reproducing, exchanging, comparing, and building on each other's work is foundational to technological advances. Advancing biotechnology calls for reliable reuse of engineered organisms. Reliable reuse of engineered organisms requires reproducible growth and productivity. Here, we identify the experimental factors that have the greatest effect on the growth and productivity of our engineered organisms in order to demonstrate reproducibility for biotechnology. We present a draft of a Minimum Information Standard for Engineered Organism Experiments (MIEO) based on this method. We evaluate the effect of 22 factors on Escherichia coli engineered to produce the small molecule lycopene, and 18 factors on E. coli engineered to produce red fluorescent protein. Container geometry and shaking have the greatest effect on product titer and yield. We reproduce our results under two different conditions of reproducibility: conditions of use (different fractional factorial experiments), and time (48 biological replicates performed on 12 different days over 4 months).