Three-dimensional ultrasound biomicroscopy for xenograft growth analysis
Three-dimensional ultrasound biomicroscopy for xenograft growth analysis
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DOI：
10.1016/j.ultrasmedbio.2005.03.003
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发表时间：
2005-06-01
影响因子：
2.9
通讯作者：
Foster, FS
中科院分区：
文献类型：
--
作者：
Cheung, AMY;Brown, AS;Foster, FS
我们报道了在非侵入性纵向测定中使用高频超声生物显微镜（UBM）对带有黑色素瘤异种移植物的小鼠的早期肿瘤生长进行定量分析。最初，使用屏幕上的 UBM 卡尺实时测量肿瘤宽度、深度和长度，并使用标准椭球公式 w d l pi/6 计算肿瘤体积。我们能够检测到起始的微小肿瘤结节，检测下限约为 0.01 mm(3) 体积。还获得了包含这些肿瘤完整长度的连续平行横截面 UBM 图像（33 μm 步长）并将其重建为 3-D 表示。随后的分割体积分析提供了肿瘤体积的测量。当研究所有 33 个异种移植物时，使用两种技术测量的体积高度相关（r = 0.9813，p < 0.0001），并且与早期小肿瘤子集测量的相关性较低（r = 0.7973，n = 16，p = 0.0004）。进一步的分析表明，对于大多数早期发育的异种移植物，3D 分割体积分析产生的体积估计值通常小于卡尺和公式计算的值。因此，3-D UBM 成像和分割预计对于观察到以椭圆体以外的不规则形状生长的小肿瘤特别有价值。 （电子邮件：acheung@sten.sunnybrook.utoronto.ca） (c) 2005 年世界超声医学与生物学联合会。
We reported the use of high-frequency ultrasound biomicroscopy (UBM) in the quantitative analysis of early tumor growth in mice bearing melanoma xenografts in a noninvasive longitudinal assay. Initially, measurements of tumor width, depth and length were obtained using on-screen UBM calipers in real time and tumor volume was calculated with the standard ellipsoid formula w d l pi/6. We were able to detect initiating minute tumor nodules, with the lower limit of detection at approximate to 0.01 mm(3) in volume. Successive parallel cross-sectional UBM images (33 mu m step) encompassing the complete length of these tumors were also obtained and reconstructed into 3-D representations. Subsequent segmentational volumetric analysis provided a measure of tumor volume. Volume measurements using the two techniques were highly correlated when all 33 xenografts were studied (r = 0.9813, p < 0.0001) and a lower degree of correlation was measured with a subset of early small tumors (r = 0.7973, n = 16, p = 0.0004). Further analysis demonstrated that 3-D segmentational volumetric analysis yielded volume estimates that were often smaller than the caliper-and-formula calculation for most early developing xenografts. Thus, 3-D UBM imaging and segmentation is expected to be especially valuable for small tumors that were observed to grow in irregular shapes other than ellipsoids. (E-mail: acheung@sten.sunnybrook.utoronto.ca) (c) 2005 World Federation for Ultrasound in Medicine & Biology.