siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference
siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference
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DOI：
10.1093/nar/gkh442
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发表时间：
2004-07-01
影响因子：
14.9
通讯作者：
Saigo, K
中科院分区：
文献类型：
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作者：
Naito, Y;Yamada, T;Saigo, K
siDirect（http：//design.RNAi.jp/）是一个基于网络的在线软件系统，用于计算具有最大靶特异性的哺乳动物RNA干扰（RNAi）的高效小干扰RNA（siRNA）序列。使用通过广泛研究siRNA序列和RNAi活性之间的关系而建立的新指南来选择高效siRNA序列。我们的高效软件避免了脱靶基因沉默，以枚举广泛使用的BLAST搜索可能忽略的潜在交叉杂交候选人。该网站接受任意序列作为输入，并快速返回siRNA候选者，在哺乳动物RNAi中提供广泛的应用，包括系统功能基因组学和治疗性基因沉默。
siDirect (http://design.RNAi.jp/) is a web-based online software system for computing highly effective small interfering RNA (siRNA) sequences with maximum target-specificity for mammalian RNA interference (RNAi). Highly effective siRNA sequences are selected using novel guidelines that were established through an extensive study of the relationship between siRNA sequences and RNAi activity. Our efficient software avoids off-target gene silencing to enumerate potential cross-hybridization candidates that the widely used BLAST search may overlook. The website accepts an arbitrary sequence as input and quickly returns siRNA candidates, providing a wide scope of applications in mammalian RNAi, including systematic functional genomics and therapeutic gene silencing.