Structural and Functional Analysis of the Gut Microbiome for Toxicologists.
Structural and Functional Analysis of the Gut Microbiome for Toxicologists.
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毒理学家的肠道微生物组的结构和功能分析。
DOI：
10.1002/cptx.54
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发表时间：
2018-11
期刊：
影响因子：
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通讯作者：
Patterson AD
中科院分区：
文献类型：
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作者：
Nichols RG;Cai J;Murray IA;Koo I;Smith PB;Perdew GH;Patterson AD
表征毒物、肠道微生物群和宿主之间的相互作用，对于提高我们对毒性终点的机械理解具有很大的希望。非培养物依赖性测序分析的进展（例如，16 S rRNA基因扩增子测序）结合定量代谢物分析（即，代谢组学）提供了研究肠道微生物组的新方法，并开始阐明毒物如何影响肠道微生物组的结构和功能。制定一个标准化的方案对于建立可靠的、可重复的、重要的是可比较的数据非常重要。该方案可用作通过基于测序的分析和代谢组学检查肠道微生物组的基础。两个主要单位如下：（1）通过基于测序的方法分析肠道微生物组;和（2）通过代谢组学分析肠道微生物组的功能。
Characterizing the reciprocal interactions between toxicants, the gut microbiota, and the host, holds great promise for improving our mechanistic understanding of toxic endpoints. Advances in culture-independent sequencing analysis (e.g., 16S rRNA gene amplicon sequencing) combined with quantitative metabolite profiling (i.e., metabolomics) have provided new ways of studying the gut microbiome and have begun to illuminate how toxicants influence the structure and function of the gut microbiome. Developing a standardized protocol is important for establishing robust, reproducible, and importantly, comparative data. This protocol can be used as a foundation for examining the gut microbiome via sequencing-based analysis and metabolomics. Two main units follow: (1) analysis of the gut microbiome via sequencing-based approaches; and (2) functional analysis of the gut microbiome via metabolomics.