Prevalence and characteristics of Shiga toxin-producing Escherichia coli isolated from retail raw meats in China
Prevalence and characteristics of Shiga toxin-producing Escherichia coli isolated from retail raw meats in China
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中国零售生肉中产志贺毒素大肠杆菌的流行情况及特征
DOI：
10.1016/j.ijfoodmicro.2015.01.018
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发表时间：
2015-05-04
影响因子：
5.4
通讯作者：
Xiong, Yanwen
中科院分区：
文献类型：
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作者：
Bai, Xiangning;Wang, Hong;Xiong, Yanwen
产志贺毒素的大肠杆菌（STEC）引起人类腹泻、出血性结肠炎和溶血性尿毒症综合征。大多数人类感染是由于食用了被大肠杆菌污染的动物源性食品。在这项研究中，我们评估了从中国两个地理区域收集的零售生肉中产志贺毒素大肠杆菌的患病率。结果显示，853个样本中有166个呈阳性；从58份stx阳性样品中分离出63株STEC，分别为猪肉（4.4%,14/318）、牛肉（11.0%,21/191）、羊肉（20.6%,26/126）、鸡肉（0.5%,1/205）和鸭肉（7.7%,1/13）。共鉴定出26个O型血清和33个O:H型血清。63株STEC分离株中均检测到3种stx(1)亚型和5种stx(2)亚型（2a ~ 2e），其中stx（2e）阳性STEC分离株最多（39.7%），其次为stx（1c）单独（20.6%）、stx(1c) + stx（2b）单独（143%）和stx（1a）单独（9.5%）。产志贺毒素大肠杆菌分离株携带毒力基因eae（6.3%）、ehxA（36.5%）、katP（4.8%）、astA（11.1%）和subA（36.5%）。在检测的四种粘附相关基因中，toxB不存在，而saa、paa和efa1分别存在于28株、3株和1株STEC分离株中。分离菌株分为50种PFGE模式和33种序列类型。通过PFGE和MIST对不同来源和地理区域的产志异大肠杆菌进行了分离。我们的研究结果显示，零售生肉中产大肠杆菌的遗传多样性很高，其中一些有可能导致人类疾病。(C) 2015 Elsevier B.V.版权所有
Shiga toxin-producing Escherichia colt (STEC) causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome in humans. Most human infections are attributed to consumption of STEC-contaminated foodstuffs of animal origin. In this study, we evaluated the prevalence of STEC from retail raw meats collected from two geographical regions in China. The results revealed that 166 out of 853 samples were stx-positive; 63 STEC isolates were recovered from 58 stx-positive samples including pork (4.4%, 14/318), beef (11.0%, 21/191), mutton (20.6%, 26/126), chicken (0.5%, 1/205), and duck (7.7%, 1/13). Twenty-six O serogroups and 33 O:H serotypes were identified. All three stx(1) subtypes and five stx(2) subtypes (2a to 2e) were found in the 63 STEC isolates, among which stx(2e)-positive STEC isolates were the most predominant (39.7%), followed by stx(1c) only (20.6%), stx(1c) + stx(2b) (143%), and stx(1a) only (9.5%). STEC isolates carried virulence genes eae (6.3%), ehxA (36.5%), katP (4.8%), astA (11.1%), and subA (36.5%). Of the four adherence-associated genes tested, toxB was absent, whereas saa, paa, and efa1 were present in 28, three, and one STEC isolates respectively. The STEC isolates were divided into 50 PFGE patterns and 33 sequence types. STEC from different sources and geographical regions were separated by PFGE and MIST. Our results revealed that there is a high genetic diversity of STEC in retail raw meats, some of which have potential to cause human diseases. (C) 2015 Elsevier B.V. All rights reserved.