A1 CELL GROUP MEDIATES SOLITARY NUCLEUS EXCITATION OF SUPRAOPTIC VASOPRESSIN CELLS
A1 CELL GROUP MEDIATES SOLITARY NUCLEUS EXCITATION OF SUPRAOPTIC VASOPRESSIN CELLS
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DOI：
10.1152/ajpregu.1989.257.5.r1020
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发表时间：
1989-11-01
影响因子：
--
通讯作者：
SIBBALD, JR
中科院分区：
文献类型：
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作者：
DAY, TA;SIBBALD, JR
刺激孤束核（NTS）可通过下丘脑的儿茶酚胺能投射兴奋视上核（SON）的加压素分泌细胞。尽管最近的证据表明，从NTS到SON的直接儿茶酚胺能投射，我们已经进行了单单位记录实验，在戊巴比妥钠麻醉大鼠的可能性，NTS刺激SON加压素细胞的影响是间接的，通过A1去甲肾上腺素能细胞群的尾侧延髓腹外侧中继。比较了单脉冲NTS和A1区刺激对逆行鉴定的SON神经分泌细胞活性的影响，SON神经分泌细胞的功能特征是分泌加压素。NTS刺激激发了81%的所有假定的加压素分泌细胞（n = 83），平均起始潜伏期为51 +/- 1 ms。A1区刺激激发了76%的所有测试细胞和90%的对NTS刺激有反应的单位，平均潜伏期为39 +/-1ms。与先前的工作一致，NTS刺激仅激发少数测试的催产素细胞（3/13），其中三分之二对A1刺激也有反应。双侧A1区的电解损伤取消了NTS刺激对假定的加压素细胞的影响。同侧A1区注射抑制性神经递质γ-氨基丁酸可逆地阻断了NTS刺激对侧A1区已经受损的动物中推定的加压素细胞的作用。这些结果支持的建议，刺激后的SON加压素分泌细胞的NTS是由于激活通过尾侧延髓腹外侧区的A1去甲肾上腺素能细胞群的中继投射。
Stimulation of the nucleus tractus solitarius (NTS) excites putative vasopressin-secreting cells of the supraoptic nucleus (SON) via a catecholaminergic projection to hypothalamus. Despite recent evidence of a direct catecholaminergic projection from NTS to SON, we have performed single-unit recording experiments in pentobarbital sodium-anesthetized rats to investigate the possibility that NTS stimulation effects on SON vasopressin cells are indirect, being relayed via the A1 noradrenergic cell group of the caudal ventrolateral medulla. The effects of single-pulse NTS and A1 region stimulation on the activity of antidromically identified SON neurosecretory cells that had been functionally characterized as vasopressin secreting were compared. NTS stimulation excited 81% of all putative vasopressin-secreting cells tested (n = 83), with a mean onset latency of 51 +/- 1 ms. A1 region stimulation excited 76% of all cells tested and 90% of units responsive to NTS stimulation, with a mean latency of 39 +/- 1 ms. Consistent with previous work NTS stimulation excited only a minority of oxytocin cells tested (3/13), and of these two-thirds also responded to A1 stimulation. Bilateral electrolytic lesions of the A1 region abolished the effects of NTS stimulation on putative vasopressin cells. Ipsilateral A1 region injections of the inhibitory neurotransmitter gamma-amino-butyric acid reversibly blocked NTS stimulation effects on putative vasopressin cells in animals where the contralateral A1 region had already been lesioned. These results support the proposal that excitation of SON vasopressin-secreting cells after NTS stimulation is due to activation of a relay projection through the A1 noradrenergic cell group of the caudal ventrolateral medulla.