Affibody-displaying bio-nanocapsules effective in EGFR, typical biomarker, expressed in various cancer cells
Affibody-displaying bio-nanocapsules effective in EGFR, typical biomarker, expressed in various cancer cells
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DOI：
10.1016/j.bmcl.2016.11.038
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发表时间：
2017-01-15
影响因子：
2.7
通讯作者：
Kondo, Akihiko
中科院分区：
文献类型：
--
作者：
Nishimura, Yuya;Ezawa, Ryosuke;Kondo, Akihiko
表皮生长因子受体（EGFR）在多种肿瘤细胞中的表达因其在药物递送系统中用作肿瘤标志物而引起人们的关注。因此，已经开发出具有靶向EGFR能力的结合分子。其中，我们重点关注源自葡萄球菌蛋白 A 的结合蛋白的亲和体。通过在源自乙型肝炎病毒 (HBV) 的生物纳米胶囊 (BNC) 表面展示与 EGFR 结合的亲和体 (Z(EGFR))，我们开发了一种对 EGFR 表达细胞具有高度特异性的改变的 BNC (Z(EGFR)-BNC)。我们考虑了两种不同类型的 Z(EGFR)（Z955 和 Z1907），发现显示 Z1907 二聚体的 BNC（[Z1907[(2)-BNC）可以有效地与表达 EGFR 的细胞结合并将药物递送至胞质溶胶。由于本研究表明[Z1907](2)-BNC可以靶向表达EGFR的细胞，因此我们将使用该颗粒作为表达EGFR的各种癌细胞的药物递送载体。 (C) 2016 年作者。由 Elsevier Ltd 出版。这是一篇遵循 CC BY-NC-ND 许可的开放获取文章。
The expression of epidermal growth factor receptor (EGFR) across a wide range of tumor cells has attracted attention for use as a tumor marker in drug delivery systems. Therefore, binding molecules with the ability to target EGFR have been developed. Among them, we focused on affibodies that are binding proteins derived from staphylococcal protein A. By displaying affibody (Z(EGFR)) binding to EGFR on the surface of a bio-nanocapsule (BNC) derived from a hepatitis B virus (HBV), we developed an altered BNC (Z(EGFR)-BNC) with a high specificity to EGFR-expressing cells. We considered two different types of Z(EGFR) (Z955 and Z1907), and found that the Z1907 dimer-displaying BNC ([Z1907[(2)-BNC) could effectively bind to EGFR-expressing cells and deliver drugs to the cytosol. Since this study showed that [Z1907](2)-BNC could target EGFR-expressing cells, we would use this particle as a drug delivery carrier for various cancer cells expressing EGFR. (C) 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license.