Expression of wild-type p53-induced phosphatase 1 in diabetic epiretinal membranes.
Expression of wild-type p53-induced phosphatase 1 in diabetic epiretinal membranes.
复制标题
野生型 p53 诱导的磷酸酶 1 在糖尿病视网膜前膜中的表达
DOI：
10.18632/oncotarget.16683
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发表时间：
2017-05-30
期刊：
影响因子：
--
通讯作者：
Xu F
中科院分区：
文献类型：
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作者：
Xu J;Zhong H;Cui L;Lan Q;Chen L;He W;Wu Y;Jiang L;Huang H;Zhao X;Li L;Zeng S;Li M;Xu F
目的探讨野生型P53诱导的磷酸酶1(WIP1)在伴有视网膜前膜(ERM)的增殖性糖尿病视网膜病变(PDR)中的表达和分布，分析WIP1与核因子-kappaB(NF-κB)p65在ERM中的共存情况。方法收集PDR患者(PDR组)和非糖尿病特发性视网膜前膜(IERM)患者(对照组)玻璃体切割术中视网膜前膜标本。实时荧光定量聚合酶链式反应(Real-time PCR，RT-PCR)检测ERM中Wip1基因的表达。免疫组织化学和免疫荧光分析检测Wip1蛋白在ERM中的表达。免疫荧光双标记法检测Wip1与视网膜胶质细胞标记物胶质纤维酸性蛋白(GFAP)以及Wip1和NF-κB的共存情况。结果视网膜脱离组17例17眼，非糖尿病组9例9眼。我们的结果显示，PDR后，Wip1mRNAs在ERM中高表达，而在iERM中低表达。此外，免疫组织化学和免疫荧光分析均显示PDR ERMS中Wip1呈强免疫反应。此外，Wip1和GFAP在PDR膜上共表达。Wip1的表达与NF-κB的表达呈平行关系。结论Wip1通过NF-κB信号参与了视网膜色素上皮细胞膜ERM的形成。
Objective The aims of the present study were to investigate the expression and distribution of Wild-type p53-induced phosphatase 1 (Wip1) in diabetic patients with proliferative diabetic retinopathy (PDR) with epiretinal membranes (ERMs) meanwhile analyze the colocalization of Wip1 and nuclear factor kappa-B (NF-κB) p65 in ERMs. Methods ERMs samples were collected from patients with PDR (PDR group) or non-diabetic patients with idiopathic epiretinal membranes (iERMs) (control group) during pars plana vitrectomy. Real-Time PCR analysis was carried out to examine the mRNA expression of Wip1 in ERMs. Immunohistochemical analysis and Immunofluorescent analysis were performed to detect the protein expression of Wip1 in ERMs. Double immunofluorescent staining was performed to detect the colocalization of Wip1 and glial fibrillary acidic protein (GFAP) (retinal glial cells marker), also Wip1 and NF-κB. Results ERMs were obtained from 17 eyes of 17 patients with PDR (the PDR group) and 9 eyes of 9 nondiabetic patients (the control group) with iERMs. Our results showed high expression levels of Wip1 mRNAs in ERMs after PDR, but low in iERMs. In addition, both immunohistochemistry and immunofluorescence assay showed strong immunoreactivity for Wip1 in PDR ERMs. Furthermore, Wip1 and GFAP were coexpressed in PDR membranes. Finally, the expression of Wip1 was paralleled with NF-κB. Conclusion These data support the notion that Wip1 contributes to the formation of the ERMs in PDR membranes via NF-κB signaling.