Transcriptomic analysis reveals the regulatory module of apple (Malus x domestica) floral transition in response to 6-BA
Transcriptomic analysis reveals the regulatory module of apple (Malus x domestica) floral transition in response to 6-BA
复制标题
转录组分析揭示了苹果 (Malus x Domestica) 花转变响应 6-BA 的调控模块
DOI：
10.1186/s12870-019-1695-0
复制
发表时间：
2019-03-06
影响因子：
5.3
通讯作者：
Xing, Libo
中科院分区：
文献类型：
--
作者：
Li, Youmei;Zhang, Dong;Xing, Libo
背景富士苹果园花芽产量不足是一个棘手的问题。虽然细胞分裂素（CK）促进花芽形成在苹果树，鲜为人知的机制，调节这种phenomenon.ResultsIn本研究中，高通量RNA测序（RNA-Seq）的长富2号'芽进行表征的转录响应6-BA处理在花转变的关键时期。对差异表达基因进行加权基因共表达网络分析（WGCNA），确定了激素信号转导途径，共有84个基因与开花时间基因的表达模式高度相关。CK信号成分和赤霉素（GA）信号抑制子的上调被发现有助于促进花转变。与未处理的芽相比，在6-BA处理的芽中，一系列糖代谢和信号相关基因与成花诱导期间相对高水平的蔗糖、果糖和葡萄糖相关。几个转录因子（即SPLs，SOC 1，FD和COL）参与GA，老化，光周期调节开花途径也上调6-BA治疗。此外，还分析了整合CK信号的转录因子在苹果成花诱导中的作用;包括植物色素相互作用因子（PIF 1，3），Wuschel相关同源异型盒（WOX 3，1,3），结论本研究揭示了6-BA对苹果成花和发育相关途径及转录因子的影响。它扩展了我们的知识与CK调节花在苹果树的转变的基本机制。
BackgroundInsufficient production of flower buds is an intractable problem in Fuji' apple orchards. Although cytokinin (CK) promotes flower bud formation in apple trees, little is known about the mechanisms regulating this phenomenon.ResultsIn the present study, high-throughput RNA sequencing (RNA-Seq) of Nagafu No. 2' buds was conducted to characterize the transcriptional response to 6-BA treatment during key period of floral transition. A weighted gene co-expression network analysis (WGCNA) of the differentially expressed genes identified hormone signal transduction pathways, totaling 84 genes were highly correlated with the expression pattern of flowering-time genes. The up-regulation of CK signal components and a gibberellin (GA) signal repressor were found to contribute to the promotion of floral transition. In relative comparison to non-treated buds, a series of sugar metabolism- and signal- related genes were associated with relatively high levels of sucrose, fructose, and glucose during floral induction in the 6-BA treated buds. Several transcription factors (i.e. SPLs, SOC1, FD, and COL) that are involved in GA, aging, and photoperiod-regulated flowering pathways were also upregulated by the 6-BA treatment. In addition, potential transcription factors integrating CK signaling to trigger floral induction in apple were also assessed; including PHYTO-CHROME-INTERACTING FACTOR (PIF1,3), WUSCHEL-related homeobox (WOX3,13), and CK response regulators (ARR2).ConclusionsThe present study provides insight into the response of flowering and development-related pathways and transcription factors to 6-BA during the period of floral transition in apple. It extends our knowledge of the fundamental mechanisms associated with CK-regulated floral transition in apple trees.