Expression of human antibodies in eukaryotic micro-algae
Expression of human antibodies in eukaryotic micro-algae
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DOI：
10.1016/j.vaccine.2004.11.013
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发表时间：
2005-03-07
期刊：
影响因子：
5.5
通讯作者：
Franklin, SE
中科院分区：
文献类型：
--
作者：
Mayfield, SP;Franklin, SE
在过去的十年中，基于蛋白质的疗法取得了巨大的成功。不幸的是，由于使用哺乳动物细胞发酵的资本化和生产固有的高成本，这种临床成功带来了沉重的代价。为了解决这个问题，我们已经开始开发一种在单细胞真核绿藻莱茵衣藻中表达重组蛋白的系统，从而产生人 IgA 单链抗体。莱茵衣藻中人单克隆抗体的表达为传统的基于哺乳动物的表达系统提供了一种有吸引力的替代方案，原因有几个，包括快速获得稳定质体和核转化体的能力，以及固有的低成本资本化和生产成本。 (c) 2004 Elsevier Ltd. 保留所有权利。
Protein based therapeutics have enjoyed great success over the past decade. Unfortunately, with this clinical success comes a heavy price tag, owing to the inherently high costs of capitalization and production using mammalian cell fermentation. To address this problem, we have begun developing a system for the expression of recombinant proteins in the unicellular eukaryotic green algae, Chlamydomonas reinhardtii, leading to the production of human IgA single chain antibodies.The expression of human monoclonal antibodies in C. reinhardtii offers an attractive alternative to traditional mammalian based expression systems for several reasons, including an ability to rapidly obtain stable plastid and nuclear transformants, coupled with inherently low costs of capitalization and production. (c) 2004 Elsevier Ltd. All rights reserved.