A smart sensor for monitoring antimicrobial interventions in wound infections
A smart sensor for monitoring antimicrobial interventions in wound infections
复制标题
DOI：
10.1016/j.snb.2023.135179
复制
发表时间：
2024-04-15
影响因子：
8.4
通讯作者：
Gilmore，Brendan F.
中科院分区：
文献类型：
--
作者：
Magee，Erin;Yusufu，Dilidaer;Gilmore，Brendan F.
伤口感染的有效治疗监测对于伤口护理至关重要。我们探索了一种新的方法，使用先前描述的3D打印的比色指示剂来非侵入性地监测伤口感染中的抗菌干预。该指示剂含有一种pH敏感染料，通过将颜色从蓝色变为黄色来检测伤口病原体产生的二氧化碳。该传感器在密封的96孔板中准确地报告了抗生素对铜绿假单胞菌的最小抑菌浓度。亚抑菌抗生素浓度导致传感器改变颜色，而对于抑制浓度保持蓝色。该传感器被应用于具有铜绿假单胞菌生物膜的猪皮肤伤口模型，测试几种抗微生物治疗：抗生素单一疗法、噬菌体单一疗法以及两者的组合。治疗后，与伤口密封的传感器能够对抗菌功效进行视觉监测。通过比较处理后生物负载和传感器响应，P的对数倍数降低更大。抗微生物处理后的需氧菌与较长的传感器响应时间相关。监测未成功处理的生物膜的传感器改变颜色，而在最小生物膜根除浓度或以上处理的那些没有引起颜色变化，指示P。空气污染根除该传感器有望监测伤口感染治疗效果，改善患者预后，并作为非侵入性即时护理工具促进伤口愈合。
Effective therapeutic monitoring of wound infections is crucial for wound care. We explore a novel approach using a previously described 3D-printed, colourimetric indicator to non-invasively monitor antimicrobial interventions in wound infection. The indicator contains a pH-sensitive dye which detects carbon dioxide production from wound pathogens by changing colour from blue to yellow. The sensor accurately reported the minimum inhibitory concentration of antibiotic against Pseudomonas aeruginosa in a sealed 96-well plate. Subinhibitory antibiotic concentrations resulted in the sensor changing colour, whilst remaining blue for inhibitory concentrations. The sensor was applied to a porcine skin wound model with Pseudomonas aeruginosa biofilms, testing several antimicrobial treatments: antibiotic monotherapy, phage monotherapy, and a combination of both. After treatment, the sensor, sealed with the wound, enabled visual monitoring of antimicrobial efficacy. Through comparing post-treatment bioburden and sensor response, greater log-fold reductions inP. aeruginosafollowing antimicrobial treatment correlated with longer sensor response times. Sensors monitoring unsuccessfully treated biofilms changed colour, while those treated at or above the minimum biofilm eradication concentration elicited no colour change, indicatingP. aeruginosaeradication. This sensor holds promise for monitoring wound infection treatment efficacy, improving patient outcomes, and promoting undisturbed wound healing as a non-invasive point-of-care tool.