Role of ribosome and translocon complex during folding of influenza hemagglutinin in the endoplasmic reticulum of living cells
Role of ribosome and translocon complex during folding of influenza hemagglutinin in the endoplasmic reticulum of living cells
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DOI：
10.1091/mbc.11.2.765
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发表时间：
2000-02-01
影响因子：
3.3
通讯作者：
Helenius, A
中科院分区：
文献类型：
--
作者：
Chen, W;Helenius, A
活细胞中的蛋白质折叠开始共翻译。为了分析核糖体和转运子复合体在转运到内质网过程中如何影响它，我们表达了一个带有C端延伸的突变的流感血凝素(一种I型膜糖蛋白)。二维SDS-PAGE分析表明，核糖体的附着对胞外结构域折叠或三聚体组装几乎没有影响。然而，只要链是核糖体结合的，并且在转位子复合体内部，二硫键的形成被部分抑制，三聚被抑制，蛋白质被保护而不被聚集。
Protein folding in the living cell begins cotranslationally. To analyze how it is influenced by the ribosome and by the translocon complex during translocation into the endoplasmic reticulum, we expressed a mutant influenza hemagglutinin (a type I membrane glycoprotein) with a C-terminal extension. Analysis of the nascent chains by two-dimensional SDS-PAGE showed that ribosome attachment as such had little effect on ectodomain folding or trimer assembly. However, as long as the chains were ribosome bound and inside the translocon complex, formation of disulfides was partially suppressed, trimerization was inhibited, and the protein protected against aggregation.