2,3-Butanediol Production by Acetogenic Bacteria, an Alternative Route to Chemical Synthesis, Using Industrial Waste Gas
2,3-Butanediol Production by Acetogenic Bacteria, an Alternative Route to Chemical Synthesis, Using Industrial Waste Gas
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DOI：
10.1128/aem.00355-11
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发表时间：
2011-08-01
影响因子：
4.4
通讯作者：
Simpson, Sean D.
中科院分区：
文献类型：
--
作者：
Koepke, Michael;Mihalcea, Christophe;Simpson, Sean D.
2，3-丁烷（23 BD）是一种高价值的化学品，通常由石油化工生产，但也可以由一些细菌合成。迄今为止，在依赖糖作为产物合成的碳源和能源的发酵系统中使用病原菌已经观察到最佳的微生物23 BD生产率。在这里，我们提出的证据，23 BD生产的三个非致病性产乙酸梭菌物种-自产乙醇梭菌，C。ljungdahlii和C.使用含一氧化碳的工业废气或合成气作为碳和能量的唯一来源。通过对C. ljungdahlii基因组中，从一氧化碳到23 BD的完整途径已经提出。同源基因参与这一途径也证实了其他两个物种的调查。一项基因表达研究表明，23 BD生物合成基因的mRNA积累与23 BD生产的发生之间存在相关性，而一项更广泛的伍德-扬达尔途径基因表达研究提供了一个最古老的现有生化途径之一的转录水平视图。
2,3-Butanediol (23BD) is a high-value chemical usually produced petrochemically but which can also be synthesized by some bacteria. To date, the best microbial 23BD production rates have been observed using pathogenic bacteria in fermentation systems that depend on sugars as the carbon and energy sources for product synthesis. Here we present evidence of 23BD production by three nonpathogenic acetogenic Clostridium species-Clostridium autoethanogenum, C. ljungdahlii, and C. ragsdalei-using carbon monoxide-containing industrial waste gases or syngas as the sole source of carbon and energy. Through an analysis of the C. ljungdahlii genome, the complete pathway from carbon monoxide to 23BD has been proposed. Homologues of the genes involved in this pathway were also confirmed for the other two species investigated. A gene expression study demonstrates a correlation between mRNA accumulation from 23BD biosynthetic genes and the onset of 23BD production, while a broader expression study of Wood-Ljungdahl pathway genes provides a transcription-level view of one of the oldest existing biochemical pathways.