Subsurface Imaging of Cell Organelles by Force Microscopy
Subsurface Imaging of Cell Organelles by Force Microscopy
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DOI：
10.1021/acsnano.9b04808
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发表时间：
2019-08-01
期刊：
影响因子：
17.1
通讯作者：
Garcia, Ricardo
中科院分区：
文献类型：
--
作者：
Guerrero, Carlos R.;Garcia, Pablo D.;Garcia, Ricardo
活细胞的高分辨率、无标记、非侵入性和亚表面显微镜方法的发展仍然是一个令人敬畏的问题。基于力显微镜的硬度测量有助于我们对单细胞纳米力学的理解。细胞外部结构的弹性性质，如质膜和肌动蛋白细胞骨架，主导着硬度测量，这反过来又阻碍了细胞内结构的成像。我们建议，上述限制可以通过组合单元的粘弹性属性的2D截面来克服。我们展示了外层细胞的细胞骨架和细胞核内细胞器的同时成像。相互作用力的弹性部分携带着细胞外部元素，如皮质和肌动蛋白细胞骨架的信息。非弹性分量对核仁等内部结构的流体动力阻力很敏感。
The development of high-resolution, label free, noninvasive, and subsurface microscopy methods of living cells remains a formidable problem. Force-microscopy-based stiffness measurements contribute to our understanding of single-cell nanomechanics. The elastic properties of the cell's outer structures, such as the plasma membrane and actin cytoskeleton, dominate stiffness measurements, which in turns prevents the imaging of intracellular structures. We propose that the above limitation could be overcome by combining 2D sections of the cell's viscoelastic properties. We show the simultaneous imaging of the outer cell's cytoskeleton and the organelles inside the nucleus. The elastic component of interaction force carries information on the cell's outer elements as the cortex and the actin cytoskeleton. The inelastic component is sensitive to the hydrodynamic drag of the inner structures such the nucleoli.