The transcription factor FvHmg1 negatively regulates fruiting body development in Winter Mushroom Flammulina velutipes
The transcription factor FvHmg1 negatively regulates fruiting body development in Winter Mushroom Flammulina velutipes
复制标题
转录因子FvHmg1负调控冬菇金针菇子实体发育
DOI：
10.1016/j.gene.2021.145618
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发表时间：
2021-04-05
期刊：
影响因子：
3.5
通讯作者：
Wang, Wei
中科院分区：
文献类型：
--
作者：
Meng, Li;Lyu, Xiaomeng;Wang, Wei
伞菌纲子实体的形成是真菌中最复杂、最不清楚的过程。交配型途径（matA和matB）和转录因子是该过程中的重要调节因子。在这里，我们报告了一个新的高迁移率族（HMG）框结构域蛋白FvHmg 1，作为一个负转录调节子实体的发展在冬季蘑菇金针菇。而Fvhmg 1在双核期和原始期的表达明显低于单核期。Fvhmg 1-RNAi突变体的出菇能力明显高于野生型菌株。Fvhmg 1的表达受matA和matB基因的调控，matA基因能提高Fvhmg 1的表达量，matB基因则相反。这说明当两个matA和matB基因相容的单核体杂交时，负转录因子FvHmg 1被抑制，能够形成和发育正常的完整子实体。FvHmg 1与交配型途径的关系将有助于进一步了解食用菌有性生殖和子实体发育。
Fruiting body formation in Agaricomycetes represents the most complex and unclear process in the fungi. Mating type pathways (matA and matB) and transcription factors are important regulators in the process. Here, we report a new High-mobility-group (HMG) box domain protein FvHmg1 that acts as a negative transcription regulator in fruiting body development in Winter Mushroom Flammulina velutipes. However, the expression of Fvhmg1 in dikaryon and primordial stages was significantly lower than that of monokaryon. The Fvhmg1-RNAi mutants had a better ability of fruiting than wild type strain. Overall expression of Fvhmg1 was controlled under compatible matA and matB genes where compatible matA genes could increase its expression level, while compatible matB genes had the opposite effect. It means when two monokaryons with compatible matA and matB genes were crossed, the negatively transcription factor FvHmg1 was inhibited, and normal fully fruiting body could formation and develop. The relationship between FvHmg1 and mating type pathway would advance to understand of sexual reproduction and fruiting body development in edible mushrooms.