Role of GATA-3 in IL-5 gene transcription by CD4+T cells of asthmatic patients
Role of GATA-3 in IL-5 gene transcription by CD4+T cells of asthmatic patients
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DOI：
10.1159/000085433
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Miyatake, S
中科院分区：
文献类型：
--
作者：
Kaminuma, O;Mori, A;Miyatake, S
背景：辅助性T细胞和T细胞因子在包括支气管哮喘在内的过敏性疾病中起着重要作用。我们报道了哮喘患者外周血T细胞产生IL-5的增强。转录因子加塔-3与IL-5基因表达有关。本研究旨在阐明加塔-3在哮喘患者IL-5合成上调中的作用。方法：在存在或不存在加塔-3表达载体的情况下，用IL-5启动子报告构建体及其突变体转染外周CD 4 + T细胞。比较哮喘受试者和健康供体的CD 4 + T细胞中加塔-3的信使RNA表达水平。结果如下：通过过表达加塔-3增强了CD 4 + T细胞中IL-5启动子的活性，而通过在推定的加塔-3结合位点中引入突变则降低了IL-5启动子的活性。哮喘患者CD 4 + T细胞加塔-3表达水平与健康对照组相当。结论：加塔-3的表达水平可能不是导致支气管哮喘患者IL-5高分泌的重要因素，但加塔-3参与了人外周血CD 4 + T细胞IL-5基因的转录。版权所有（C）2005 S. Karger AG，巴塞尔。
Background: Helper T cells and T cell cytokines play central roles in allergic disorders including bronchial asthma. We reported enhanced IL-5 production by peripheral blood T cells of asthmatic patients. A transcription factor, GATA-3, has been implicated in IL-5 gene expression. This study was undertaken to clarify the role of GATA-3 in the upregulation of IL-5 synthesis in asthmatic patients. Method: Peripheral CD4+ T cells were transfected with an IL-5 promoter reporter construct as well as its mutants in the presence or absence of a GATA-3 expression vector. Messenger RNA expression level of GATA-3 in CD4+ T cells of asthmatic subjects was compared to that of healthy donors. Results: IL-5 promoter activity in CD4+ T cells was enhanced by overexpression of GATA-3, whereas it was diminished by the introduction of mutations in the putative GATA-3 binding sites. The GATA-3 expression level in CD4+ T cells of asthmatic patients was equivalent to that of healthy controls. Conclusion: The expression level of GATA-3 may not be an essential factor to cause IL-5 hyperproduction in bronchial asthma, though GATA-3 is crucially involved in IL-5 gene transcription in human peripheral CD4+ T cells. Copyright (C) 2005 S. Karger AG, Basel.