ALTERATIONS IN ACETYLCHOLINE-RELEASE IN THE RAT HIPPOCAMPUS DURING SLEEP-WAKEFULNESS DETECTED BY INTRACEREBRAL DIALYSIS
ALTERATIONS IN ACETYLCHOLINE-RELEASE IN THE RAT HIPPOCAMPUS DURING SLEEP-WAKEFULNESS DETECTED BY INTRACEREBRAL DIALYSIS
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DOI：
10.1016/0024-3205(90)90300-g
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发表时间：
1990-01-01
期刊：
影响因子：
6.1
通讯作者：
KAWAMURA, H
中科院分区：
文献类型：
--
作者：
KAMETANI, H;KAWAMURA, H
使用脑内透析技术，在光照期间连续监测自由活动大鼠背侧海马区的乙酰胆碱(ACh)释放。每隔6min收集一次透析液，同时进行皮层和海马区脑电、肌电和眼电的多导描记，以确定睡眠觉醒的阶段。采用高效液相色谱-电化学检测法测定ACh含量。ACH产量表现出深刻的、与状态相关的波动。与慢波睡眠相比，清醒时的ACh水平增加了约300%。相反，在反常睡眠中，ACh的释放速率与清醒时一样高，而且似乎更高。这些结果表明，脑内透析技术为监测睡眠-觉醒周期中自发性神经递质释放的变化提供了一种有用的方法。
Acetylcholine (ACh) release from the dorsal hippocampus was continuously monitored in freely moving rats during a light period using an intracerebral dialysis technique. A dialysate was collected every 6 min and polygraph recordings including cortical and hippocampal electroencephalograms, electromyogram, and electrooculogram were simultaneously made to determine the stage of sleep-wakefulness. The content of ACh was measured by high-performance liquid chromatography with electrochemical detection. ACh output showed profound and state-dependent fluctuations. ACh levels during waking increased approximately 300% compared to slow wave sleep. In contrast, the rate of ACh release during paradoxical sleep was as high as during waking and appeared to be even higher. These results revealed that the intracerebral dialysis technique provides a useful method to monitor changes in spaontaneous neurotransmitter release during the sleep-waking cycle.