INVADING C6 GLIOMA-CELLS MAINTAINING TUMORIGENICITY
INVADING C6 GLIOMA-CELLS MAINTAINING TUMORIGENICITY
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DOI：
10.3171/jns.1995.83.4.0665
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发表时间：
1995-10-01
影响因子：
4.1
通讯作者：
SILBERGELD, DL
中科院分区：
文献类型：
--
作者：
CHICOINE, MR;SILBERGELD, DL
为了表征大鼠神经胶质瘤细胞侵袭，将2 X 10（6）荧光团标记或转染标记的C6大鼠神经胶质瘤细胞植入大鼠额叶。80%的植入大鼠形成了大块肿瘤（直径3-4 mm）。植入后两周，荧光显微镜检查显示，在距离大部分脑肿瘤16 mm以上的部位有单个肿瘤细胞。远离肿瘤块的肿瘤细胞保持单一，无肿块形成，主要沿着白色束侵入。肿瘤植入后两周，通过解聚并在1）肿瘤块、2）对侧半球和3）小脑的培养物中起始，从每个脑中产生三个细胞系;所有解聚的标本产生活的培养物。从对侧半球培养的细胞在形态上与来自大块肿瘤和来自原始C6细胞系的细胞难以区分。小脑培养的细胞在形态上与C6细胞系截然不同。将从肿瘤、对侧半球和小脑获得的分解标本的细胞植入未处理大鼠的额叶中以测试致瘤性。移植瘤组织的大鼠中有58%形成了大块肿瘤，移植对侧半球组织的大鼠中有75%形成了大块肿瘤，而移植小脑半球组织的大鼠中只有12.5%形成了大块肿瘤。在植入前用p3 ′ ssDNA载体转染标记的C6细胞进行的实验证实，从对侧半球培养的细胞来源于植入的C6细胞。用锚定在琼脂中的C6细胞进行的实验证实了向对侧半球的移动是继发于实质浸润而不是分散在脑脊液中。
To characterize rat glioma cell invasion, 2 X 10(6) fluorophore-labeled or transfection-labeled C6 rat glioma cells were implanted in the rat frontal lobe. Eighty percent of the rats implanted formed bulk tumors (3-4 mm in diameter). Two weeks after implantation, fluorescence microscopy revealed single tumor cells in sites over 16 mm from the bulk brain tumor. Tumor cells distant from the bulk tumor remained single without mass formation and invaded primarily along white matter tracts. Two weeks after tumor implantation, three cell lines were created from each brain by disaggregation and initiation in culture of 1) bulk tumor, 2) contralateral hemisphere, and 3) cerebellum; all disaggregated specimens generated viable cultures. Cells cultured from the contralateral hemisphere were morphologically indistinguishable from cells from the bulk tumor and from the original C6 cell line. Cells cultured from the cerebellum were morphologically quite distinct from the C6 cell line. Cells from disaggregated specimens obtained from the tumor, contralateral hemisphere, and cerebellum were implanted in the frontal lobe of naive rats to test tumorgenicity. Bulk tumor formed in 58% of the rats implanted with specimens from tumor, in 75% of the rats implanted with specimens from contralateral hemisphere, and in only 12.5% of the rats implanted with specimens from the cerebellar hemispheres. Experiments using C6 cells labeled by transfection with the p3'ss DNA vector prior to implantation confirmed that the cells cultured from the contralateral hemisphere were derived from the implanted C6 cells. Experiments with C6 cells anchored in agar served to verify that movement to the contralateral hemisphere was secondary to parenchymal invasion rather than dispersion in the cerebrospinal fluid.