Generation of transgenic mice and germline transmission of a mammalian artificial chromosome introduced into embryos by pronuclear microinjection
Generation of transgenic mice and germline transmission of a mammalian artificial chromosome introduced into embryos by pronuclear microinjection
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DOI：
10.1023/a:1009206926548
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发表时间：
2000-01-01
影响因子：
2.6
通讯作者：
Drayer, JI
中科院分区：
文献类型：
--
作者：
Co, DO;Borowski, AH;Drayer, JI
我们已经产生了转基因小鼠原核显微注射的小鼠卫星DNA为基础的人工染色体（SATAC）。由于50%的建立者后代是SATAC阳性的，这表明SATAC通过种系的传播已经发生。从创始人和后代的有丝分裂原激活的外周血淋巴细胞的中期染色体的FISH分析显示，SATAC被维持为一个离散的染色体，它没有整合到一个内源性染色体。据我们所知，这是第一个报告的生殖细胞传输的基因工程哺乳动物人工染色体内产生的转基因动物通过原核显微注射。我们还表明，鼠SATAC可以类似地引入牛胚胎。利用胚胎显微注射产生携带基因工程染色体的转基因哺乳动物提供了一种新的方法，通过该方法可以利用基于染色体的基因递送系统的独特优势。
We have generated transgenic mice by pronuclear microinjection of a murine satellite DNA-based artificial chromosome (SATAC). As 50% of the founder progeny were SATAC-positive, this demonstrates that SATAC transmission through the germline had occurred. FISH analyses of metaphase chromosomes from mitogen-activated peripheral blood lymphocytes from both the founder and progeny revealed that the SATAC was maintained as a discrete chromosome and that it had not integrated into an endogenous chromosome. To our knowledge, this is the first report of the germline transmission of a genetically engineered mammalian artificial chromosome within transgenic animals generated through pronuclear microinjection. We have also shown that murine SATACs can be similarly introduced into bovine embryos. The use of embryo microinjection to generate transgenic mammals carrying genetically engineered chromosomes provides a novel method by which the unique advantages of chromosome-based gene delivery systems can be exploited.