A Proteome Catalog of Drosophila melanogaster -: An essential resource for targeted quantitative proteomics
A Proteome Catalog of Drosophila melanogaster -: An essential resource for targeted quantitative proteomics
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DOI：
10.4161/fly.4532
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发表时间：
2007-05-01
期刊：
影响因子：
1.2
通讯作者：
Basler, Konrad
中科院分区：
文献类型：
--
作者：
Ahrens, Christian H.;Brunner, Erich;Basler, Konrad
蛋白质组学分析对于系统生物学是至关重要的，因为与生物系统的结构、功能和控制相关的重要方面只能通过直接的蛋白质测量来进行。很明显，目前的蛋白质组学技术不太可能允许对整个蛋白质组进行常规的定量测量。因此，我们建议并在很大程度上实施了两步定量蛋白质组分析的策略。在第一步，发现阶段，通过质谱法可观察到的蛋白质组被广泛分析。由此产生的蛋白质组目录，然后可以用来选择肽特异性只有一种蛋白质，所谓的蛋白质型肽（PTP）。它代表了在后续评分阶段基于这些PTP的靶向质谱法实现灵敏、稳健和可重现测量的基础。在这个额外的视图中，我们描述了对这种蛋白质组目录的需求及其催化向靶向定量蛋白质组学分析及其他方向转变的多重好处。我们使用胰岛素信号级联作为一个代表性的例子来说明目前使用的蛋白质组学方法的局限性，为具体的分析个别途径的组成部分，并描述如何最近出版的果蝇蛋白质组目录已经帮助克服了许多这些限制。
Proteomic analyses are critically important for systems biology because important aspects related to the structure, function and control of biological systems are only amenable by direct protein measurements. It has become apparent that the current proteomics technologies are unlikely to allow routine, quantitative measurements of whole proteomes. We have therefore suggested and largely implemented a two-step strategy for quantitative proteome analysis. In a first step, the discovery phase, the proteome observable by mass spectrometry is extensively analyzed. The resulting proteome catalog can then be used to select peptides specific to only one protein, so-called proteotypic peptides (PTPs). It represents the basis to realize sensitive, robust and reproducible measurements based on targeted mass spectrometry of these PTPs in a subsequent scoring phase. In this Extra View we describe the need for such proteome catalogs and their multiple benefits for catalyzing the shift towards targeted quantitative proteomic analysis and beyond. We use the Insulin signaling cascade as a representative example to illustrate the limitations of currently used proteomics approaches for the specific analysis of individual pathway components, and describe how the recently published Drosophila proteome catalog already helped to overcome many of these limitations.