Molecular characterization of a novel mycovirus from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi
Molecular characterization of a novel mycovirus from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi
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烟丝核菌 AG-Ba 分离株 C-314 Baishi 中新型真菌病毒的分子特征
DOI：
10.1007/s00705-015-2483-2
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发表时间：
2015-09-01
影响因子：
2.7
通讯作者：
Mo, Xiaohan
中科院分区：
文献类型：
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作者：
Li, Yanqiong;Xu, Ping;Mo, Xiaohan
测定了从烟丝核菌（Rhizoctonia fumigata）AG-Ba分离物C-314白石分离物中分离到的一种新的双链RNA病毒（Rhizoctonia fumigata virus 1，RfV 1）的全基因组序列。RfV 1基因组全长9,907 bp，含有两个开放阅读框（ORF）。ORF 1可能编码198.10-kDa蛋白（P1）。P1共享低，但显着的氨基酸序列相似性推定的蛋白质编码的香菇真菌病毒（LeV）ORF 1。P1含有一个NUDIX结构域，该结构域也存在于LeV和Phlebiopsis martea large virus 1（PgLV-1）的ORF 1编码的推定蛋白中。ORF 2可能编码一个146.72-kDa的蛋白质（P2），该蛋白质含有RNA依赖性RNA聚合酶（RdRp）的保守基序。ORF 1和ORF 2是重叠的，并且预测ORF 2可以通过核糖体-1移码机制被翻译为与ORF 1的融合体。系统进化分析表明，RfV 1与PgLV-1、LeV和Rosellinia necatrix megabirnavirus 1（RnMBV 1）聚为一个独立的分支，与其他病毒属无关。我们建议RfV 1，沿着与PgLV-1和LeV，应被归类为一个新的病毒属相关的家庭Megabirnaviridae。这是首次报道一株分离自红曲霉的新型真菌病毒的全基因组序列。烟熏。
The complete genome sequence of a novel dsRNA virus isolated from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi (designated as Rhizoctonia fumigata virus 1, RfV1) was determined. The RfV1 genome was 9,907 bp in length and contained two open reading frames (ORFs). ORF1 potentially coded for a 198.10-kDa protein (P1). P1 shared low but significant amino acid sequence similarity to the putative protein encoded by Lentinula edodes mycovirus (LeV) ORF1. P1 contained a NUDIX domain, which was also present in the putative proteins encoded by the ORF1s of LeV and Phlebiopsis gigantea large virus 1 (PgLV-1). ORF2 potentially coded for a 146.72-kDa protein (P2) that contained the conserved motifs of the RNA-dependent RNA polymerase (RdRp). ORF1 and ORF2 were overlapping, and it was predicted that ORF2 could be translated as a fusion with ORF1 via a ribosomal -1 frameshifting mechanism. Phylogenetic analysis indicated that RfV1 clustered with PgLV-1, LeV and Rosellinia necatrix megabirnavirus 1 (RnMBV1) in a separate clade independent of other virus genera. We propose that RfV1, along with PgLV-1 and LeV, should be grouped into a new viral genus related to the family Megabirnaviridae. This is the first report of the full-length genome sequence of a novel mycovirus isolated from R. fumigata.