Enzyme Immunoassay Detection of Antibodies in North American Blastomycosis: Comparison of Three Blastomyces dermatitidis Antigens/Enzymimmunoassay zum Antikörpernachweis bei Nordamerikanischer Blastomykose: Vergleich dreier Blastomyces dermatitidis‐Antigene
Enzyme Immunoassay Detection of Antibodies in North American Blastomycosis: Comparison of Three Blastomyces dermatitidis Antigens/Enzymimmunoassay zum Antikörpernachweis bei Nordamerikanischer Blastomykose: Vergleich dreier Blastomyces dermatitidis‐Antigene
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北美芽生菌病抗体的酶免疫分析检测：三种皮炎芽生菌抗原的比较/酶免疫分析 zum Antikörpernachweis bei Nordamerikanischer Blastomykose: Vergleich dreier Blastomyces dermatitidis-Antigene
DOI：
10.1111/j.1439-0507.1988.tb03650.x
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发表时间：
1988
期刊：
影响因子：
4.9
通讯作者：
G. M. Scalarone
中科院分区：
文献类型：
--
作者：
J. Wheeler;R. D. Owens;G. M. Scalarone
摘要：三种商品化皮炎芽孢杆菌免疫扩散抗原，子午线诊断，诺兰/斯科特实验室和美国Microsan，被用于酶免疫分析(ELISA)检测芽生真菌病的抗体。用碱性磷酸酶间接酶联免疫吸附试验检测了32例芽生菌病患者的血清。根据ELISA指标值，子午线诊断抗原表现出更高的反应性；其他两种抗原表现出较低的反应性，但具有可比性。通过对组织胞浆菌病患者的血清或组织胞浆菌皮肤试验阳性的个体的样本进行交叉反应分析来确定特异性。三种抗原的特异性结果具有可比性。
Summary: Three Blastomyces dermatitidis commercial immunodiffusion antigens, Meridian Diagnostics, Nolan/Scott Laboratories and American Microsan, were utilized in an enzyme immunoassay (ELISA) for the detection of antibodies in blastomycosis. Thirty two serum specimens from patients with blastomycosis were assayed by the indirect ELISA using the alkaline phosphatase enzyme system. Greater reactivity, based on the ELISA index value, was evidenced with the Meridian Diagnostics antigen; the other two antigens exhibited lower but comparable reactivity. Specificity was determined by performing cross‐reactivity assays on sera from patients with histoplasmosis or on specimens from individuals that More Histoplasma capsulatum skin test positive. Comparable specificity results were obtained with all three antigens.