Toward an in vivo neurochemical profile:: Quantification of 18 metabolites in short-echo-time 1H NMR spectra of the rat brain
Toward an in vivo neurochemical profile:: Quantification of 18 metabolites in short-echo-time 1H NMR spectra of the rat brain
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DOI：
10.1006/jmre.1999.1895
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发表时间：
1999-11-01
影响因子：
2.2
通讯作者：
Gruetter, R
中科院分区：
文献类型：
--
作者：
Pfeuffer, J;Tkác, I;Gruetter, R
在9.4 T下，用2 ms的回声时间在大鼠脑内进行局部体内H-1 NMR谱分析，LCModel频域分析表明，体内光谱可以用18个代谢物模型溶液光谱和高度结构化的背景来解释，这归因于体内T-1比代谢物短5倍的共振。高光谱分辨率（最大半宽约0.025 ppm）和灵敏度（63 μ L体积，512次扫描的信噪比约为45）用于同时测量以前在H-1光谱中难以量化的代谢物浓度。n -乙酰天冬氨酸、谷氨酸、牛磺酸、肌醇、肌酸、磷酸肌酸、谷氨酰胺和乳酸的强代表信号用Cramer-Rao下界在4%以下进行量化。胆碱组、磷酸乙醇胺组、葡萄糖组、谷胱甘肽组、γ -氨基丁酸组、n -乙酰天冬氨酸组和丙氨酸组低于13%，而天冬氨酸组和三基肌醇组低于22%。从3分钟光谱的时间序列评估测定内的变异，变异系数与计算的Cramer-Rao下界相似。从31个合并光谱中确定了测定间的变异，总肌酸的变异系数为7%。组织浓度被发现与文献中的神经化学数据非常一致。(C) 1999学术出版社。
Localized in vivo H-1 NMR spectroscopy was performed with 2-ms echo time in the rat brain at 9.4 T, Frequency domain analysis with LCModel showed that the in vivo spectra can be explained by 18 metabolite model solution spectra and a highly structured background, which was attributed to resonances with fivefold shorter in vivo T-1 than metabolites. The high spectral resolution (full width at half maximum approximately 0.025 ppm) and sensitivity (signal-to-noise ratio approximately 45 from a 63-mu L volume, 512 scans) was used for the simultaneous measurement of the concentrations of metabolites previously difficult to quantify in H-1 spectra. The strongly represented signals of N-acetylaspartate, glutamate, taurine, myo-inositol, creatine, phosphocreatine, glutamine, and lactate were quantified with Cramer-Rao lower bounds below 4%. Choline groups, phosphorylethanolamine, glucose, glutathione, gamma-aminobutyric acid, N-acetylaspartylglutamate, and alanine were below 13%, whereas aspartate and scyllo-inositol were below 22%. Intra-assay variation was assessed from a time series of 3-min spectra, and the coefficient of variation was similar to the calculated Cramer-Rao lower bounds. Interassay variation was determined from 31 pooled spectra, and the coefficient of variation for total creatine was 7%. Tissue concentrations were found to be in very good agreement with neurochemical data from the literature. (C) 1999 Academic Press.