Prevalence of Smqnr and plasmid-mediated quinolone resistance determinants in clinical isolates of Stenotrophomonas maltophilia from Japan: novel variants of Smqnr.
Prevalence of Smqnr and plasmid-mediated quinolone resistance determinants in clinical isolates of Stenotrophomonas maltophilia from Japan: novel variants of Smqnr.
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DOI：
10.1016/j.nmni.2015.04.009
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发表时间：
2015-09
影响因子：
4
通讯作者：
Kaku, M.
中科院分区：
文献类型：
--
作者：
Kanamori, H.;Yano, H.;Tanouchi, A.;Kakuta, R.;Endo, S.;Ichimura, S.;Ogawa, M.;Shimojima, M.;Inomata, S.;Ozawa, D.;Aoyagi, T.;Weber, D. J.;Kaku, M.
嗜麦芽窄食单胞菌是医疗保健相关感染的重要病原体。嗜麦芽窄食单胞菌可能含有编码五肽重复蛋白的喹诺酮耐药基因Smqnr，其在异源宿主中表达时赋予低水平的喹诺酮耐药。我们调查了来自日本的嗜麦芽窄食单胞菌分离株中Smqnr和质粒介导的喹诺酮类耐药（PMQR）决定因素的流行情况。从日本的四个地区共收集了181个连续和非重复的嗜麦芽窄食单胞菌临床分离株。测定了这些菌株的抗菌药物敏感性。对Smqnr和PMQR基因（包括qnrA、qnrB、qnrC、qnrS、aac（6′）-Ib和qepA）进行PCR检测。对Smqnr和aac（6′）-Ib的PCR产物进行测序。对于含有Smqnr的嗜麦芽窄食单胞菌分离株，使用XbaI进行脉冲场凝胶电泳（PFGE）。对头孢他啶、左氧氟沙星、复方新诺明、氯霉素和米诺环素的耐药率分别为67.4%、6.1%、17.7%、8.8%和0%。抑制50%和90%微生物生长所需的最小抑菌浓度分别为0.5和2 mg/L，而左氧氟沙星分别为1和4 mg/L。181株嗜麦芽窄食单胞菌中有104株（57.5%）检测到Smqnr，其中以Smqnr 6最常见，其次为Smqnr 8和Smqnr 11。从Smqnr 48到Smqnr 58共检测到11个新的变异体。对24株含Smqnr基因的嗜麦芽窄食单胞菌进行PFGE分型，将其分为21个独特型。9株嗜麦芽窄食单胞菌（5.0%）携带aac（6′）-Ib-cr。未检测到qnr或qepA基因。本研究描述了日本嗜麦芽窄食单胞菌中Smqnr的高患病率和Smqnr的新变体。需要对嗜麦芽窄食单胞菌的喹诺酮类耐药性进行持续监测和进一步的分子流行病学研究。
Stenotrophomonas maltophilia is an important pathogen in healthcare-associated infections. S. maltophilia may contain Smqnr, a quinolone resistance gene encoding the pentapeptide repeat protein, which confers low-level quinolone resistance upon expression in a heterologous host. We investigated the prevalence of Smqnr and plasmid-mediated quinolone resistance (PMQR) determinants in S. maltophilia isolates from Japan. A total of 181 consecutive and nonduplicate clinical isolates of S. maltophilia were collected from four areas of Japan. The antimicrobial susceptibility profiles for these strains were determined. PCR was conducted for Smqnr and PMQR genes, including qnrA, qnrB, qnrC, qnrS,aac(6′)-Ib and qepA. PCR products for Smqnr and aac(6′)-Ib were sequenced. For the S. maltophilia isolates containing Smqnr, pulsed-field gel electrophoresis (PFGE) was performed using XbaI. Resistance rates to ceftazidime, levofloxacin, trimethoprim–sulfamethoxazole, chloramphenicol and minocycline were 67.4%, 6.1%, 17.7%, 8.8% and 0%, respectively. The minimum inhibitory concentration required to inhibit the growth of 50% and 90% of organisms were 0.5 and 2 mg/L for moxifloxacin but 1 and 4 mg/L for levofloxacin, respectively. Smqnr was detected in 104 of the 181 S. maltophilia isolates (57.5%), and the most frequent was Smqnr6, followed by Smqnr8 and Smqnr11. Eleven novel variants from Smqnr48 to Smqnr58 were detected. The 24 Smqnr-containing S. maltophilia isolates were typed by PFGE and divided into 21 unique types. Nine S. maltophilia isolates (5.0%) carried aac(6′)-Ib-cr. No qnr or qepA genes were detected. This study describes a high prevalence of Smqnr and novel variants of Smqnr among S. maltophilia from Japan. Continuous antimicrobial surveillance and further molecular epidemiological studies on quinolone resistance in S. maltophilia are needed.
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影响因子：
4.9
作者：
Kim, Hong Bin;Park, Chi Hye;Hooper, David C.
通讯作者：
Hooper, David C.
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