Is hydrogen peroxide a second messenger of salicylic acid in systemic acquired resistance
Is hydrogen peroxide a second messenger of salicylic acid in systemic acquired resistance
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DOI：
10.1046/j.1365-313x.1995.08020227.x
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发表时间：
1995-08
期刊：
影响因子：
7.2
通讯作者：
Urs Neuenschwander;B. Vernooij;L. Friedrich;S. Uknes;H. Kessmann;J. Ryals
中科院分区：
文献类型：
--
作者：
Urs Neuenschwander;B. Vernooij;L. Friedrich;S. Uknes;H. Kessmann;J. Ryals
摘要提高水杨酸(SA)水平是诱导植物系统获得性抗性(SAR)所必需的。最近，从烟草中分离出的一种水杨酸结合蛋白(SABP)被证明具有柴胡活性。基于这一发现，高水平的过氧化氢(H202)被认为是SA在SAR信号转导途径中的第二信使。为了阐明H202在SAR信号转导中的作用，已经进行了一系列的实验。在急性呼吸窘迫综合征发作期间，未发现H202的升高。在表达细菌水杨酸羟基酶基因的转基因烟草植株中，H202和H20z诱导剂对SAR基因PR-1的诱导受到强烈抑制，表明H202对SAR基因的诱导依赖于SA的积累。在增加H202浓度的植物处理后，发现总SA种类的积累具有剂量依赖性，这表明H202可能通过SA积累诱导PR-1基因的表达。虽然这些结果不支持HzO2在SAR信号转导中的作用，但有人认为SA对过氧化氢酶活性的抑制在经历过敏反应的组织中可能是重要的。
Summary Elevated levels of salicylic acid (SA) are required for the induction of systemic acquired resistance (SAR) in plants. Recently, a salicylic acid-binding protein (SABP) isolated from tobacco was shown to have cetelasa activity. Based on this finding elevated levels of hydrogen peroxide (H202} were postulated to act as a second messenger of SA in the SAR signal trensduction pathway. A series of experiments have been carried out to clarify the role of H202 in SARsignaling. No increase of H202 was found during the onset of SAR. Induction of the SAR gene, PR-1, by H202 and H20 zinducing chemicals is strongly suppressed in transgenic tobacco plants that express the bacterial salicylate hydroxylase gene, indicating that H202 induction of SAR genes is dependent on SA accumulation. Following treatment of plants with increasing concentrations of H202, a dose-dependent accumulation of total SA species was found, suggesting that H202 may induce PR-1 gene expression through SA accumulation. While the results do not support a role for HzO2 in SAR signaling, it is suggested that SA inhibition of catalase activity may be important in tissues undergoing a hypersensitive response.