Characterization of a signal peptide sequence in the cell-free translation product of sheep elastin mRNA.
Characterization of a signal peptide sequence in the cell-free translation product of sheep elastin mRNA.
复制标题
绵羊弹性蛋白 mRNA 无细胞翻译产物中信号肽序列的表征。
DOI：
10.1016/0003-9861(82)90317-4
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发表时间：
1982
影响因子：
3.9
通讯作者：
L. Sandberg
中科院分区：
文献类型：
--
作者：
J. Davidson;B. Leslie;T. Wolt;R. Crystal;L. Sandberg
近期绵羊项韧带的体外外植体培养物分泌大约70,000-72,000Mr的弹性蛋白原，而绵羊颈项韧带RNA的弹性蛋白无细胞产物则大2000至3000Mr。放射性标记的无细胞弹性蛋白前体的免疫沉淀物的自动 Edman 降解证明存在 26 个残基信号序列，而外植体培养物分泌的绵羊原弹性蛋白中不存在该信号序列。此外，在无细胞产物和分泌蛋白之间建立了 20 个残基重叠。该重叠区域代表绵羊原弹性蛋白的 N 端序列，证明与猪原弹性蛋白的 N 端序列完全同源，并且与鸡原弹性蛋白近乎完全同源。这些发现表明，这种疏水性肽延伸的共翻译去除可能与弹性蛋白向分泌装置的矢量运输有关。
In vitroexplant cultures of near-term sheep nuchal ligament secrete tropoelastin of approximateMr70,000–72,000 while the elastin cell-free product of sheep nuchal ligament RNA is 2000 to 3000Mrlarger. Automated Edman degradation of immunoprecipitates of radiolabeled cell-free elastin precursor demonstrated the presence of a 26-residue signal sequence which was absent from sheep tropoelastin secreted from explant cultures. In addition, a 20-residue overlap was established between the cell-free product and the secreted protein. This overlap region, representing the N-terminal sequence of ovine tropoelastin, demonstrated complete homology with the N-terminal sequence of porcine tropoelastin and near complete homology with chick tropoelastin. These findings suggest that cotranslational removal of this hydrophobic peptide extension is likely a correlate of vectorial transport of elastin into the secretory apparatus.