Seasonal influences on the use of genetic markers as performance indicators for small wastewater treatment plants.
Seasonal influences on the use of genetic markers as performance indicators for small wastewater treatment plants.
复制标题
季节性对使用遗传标记作为小型废水处理厂绩效指标的影响。
DOI：
10.1016/j.scitotenv.2020.139928
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
D. Graham
中科院分区：
文献类型：
--
作者：
J. Bunce;A. Robson;D. Graham
微生物来源追踪方法的发展产生了一系列遗传粪便标记物，用于评估地表水污染对人类健康造成的风险。然而，它们在废水处理厂（WWTPs）的性能指标的使用还没有得到广泛的探讨。在这里，我们比较了三个拟杆菌（HF 183，HumM 2，AllBac）和两个大肠杆菌（H8，RodA）的遗传标记，用于英格兰东北部12个小型农村（<250 PE）和3个较大的污水处理厂的夏季和冬季性能监测。小型污水处理厂之所以受到关注，是因为人们对它们了解甚少，而且它们对地表水质量的影响可能被低估。总体而言，遗传标记数据显示，在较小与较大的污水处理厂的处理性能显着差异。例如，在较小的系统中，HF 183和HumM 2的流出物丰度显著更高（HumM 2的p= 0.003; HF 183的p= 0.02）。遗传标记也显示出季节间表现的显著差异（p<0.01，n = 120），人类特异性标记（即，HF 183、HumM 2、H8）总体上更适合夏季污水处理厂监测。相比之下，拟杆菌标记物更适合冬季监测，可能是因为大肠杆菌标记物对温度和阳光条件的差异不太敏感。总体而言，Bacteroidesmarker最好地描述了所有样本的污水处理厂处理性能，尽管季节差异表明，当使用标记进行性能监测时，需要谨慎。遗传标记无疑提供了快速和新的信息污水处理厂的性能，但需要更多的空间多样性的研究，以完善其用于常规污水处理厂监测。
The development of microbial source tracking methods has resulted in an array of genetic faecal markers for assessing human health risks posed from surface water pollution. However, their use as performance metrics at wastewater treatment plants (WWTPs) has not been explored extensively. Here we compared threeBacteroides(HF183, HumM2, AllBac) and twoE.coli(H8, RodA) genetic markers for summer and winter performance monitoring at twelve small rural (<250 PE) and three larger WWTPs in NE England. Small WWTPs are of interest because they are poorly understood and their impact on surface water quality may be underestimated. Overall, genetic marker data showed significant differences in treatment performance at smaller versus larger WWTPs. For example, effluent abundances of HF183 and HumM2 were significantly higher in smaller systems (p= 0.003 for HumM2;p= 0.02 for HF183). Genetic markers also showed significant differences in performance between seasons (p< 0.01,n= 120), with human-specific markers (i.e., HF183, HumM2, H8) being generally better for summer WWTP monitoring. In contrast,Bacteroidesmarkers were much more suitable for winter monitoring, possibly because theE.colimarkers are less sensitive to differences in temperature and sunlight conditions. Overall,Bacteroidesmarkers best described WWTP treatment performance across all samples, although seasonal differences suggest caution is needed when markers are used for performance monitoring. Genetic markers definitely provide rapid and new information about WWTP performance, but more spatially diverse studies are needed to refine their use for routine WWTP monitoring.
影响因子：
12.8
作者：
Brown, M. R.;Camezuli, S.;Curtis, T. P.
通讯作者：
Curtis, T. P.