Taxonomic study of aromatic-degrading bacteria from deep-terrestrial-subsurface sediments and description of Sphingomonas aromaticivorans sp nov, Sphingomonas subterranea sp nov, and Sphingomonas stygia sp nov
Taxonomic study of aromatic-degrading bacteria from deep-terrestrial-subsurface sediments and description of Sphingomonas aromaticivorans sp nov, Sphingomonas subterranea sp nov, and Sphingomonas stygia sp nov
复制标题
DOI：
10.1099/00207713-47-1-191
复制
发表时间：
1997-01-01
期刊：
影响因子：
--
通讯作者：
Spadoni, CM
中科院分区：
文献类型：
--
作者：
Balkwill, DL;Drake, GR;Spadoni, CM
应用距离矩阵法和简约法对16 S rRNA基因序列进行系统发育分析，结果表明，从大西洋沿岸平原深层饱和沉积物中分离的6株细菌与裂殖单胞菌属（Spltingomonas）关系密切。五个菌株聚类，但不同的，鞘氨醇单胞菌capsulata，而第六个菌株是最密切相关的芽生菌。与S. capsulata都能降解芳香族化合物，它们是革兰氏阴性的、不形成孢子的、不运动的、杆状的生物体，在复合培养基上产生小的黄色菌落。它们的G + C含量范围从60.0到65.4摩尔%，和主要的类异戊二烯醌是泛醌Q-10。所有菌株均为好氧菌，过氧化氢酶阳性。吲哚，尿素酶，精氨酸水解酶没有产生。明胶不液化，葡萄糖不发酵。鞘脂存在于所有菌株; 2 OH 14：0是主要的羟基脂肪酸，和18：1是细胞脂质的主要成分。戊糖、己糖和二糖氧化生成酸，但多元醇和吲哚不能氧化生成酸。所有这些特征表明，五个芳烃降解菌株应被放置在属鞘氨醇单胞菌目前定义。系统发育分析的16 S rRNA基因序列，DNA-DNA的重新关联值，BOX-PCR基因组指纹图谱，细胞脂质组成的差异，和生理性状的差异都表明，这五个菌株代表三个以前未描述的鞘氨醇单胞菌属物种。因此，我们提出了以下新种：嗜芳香鞘氨醇单胞菌（模式菌株，SMCC P199），亚嗜芳香鞘氨醇单胞菌（模式菌株，SMCC B 0478），和斯氏鞘氨醇单胞菌（模式菌株，SMCC B 0712）。
Phylogenetic analyses of 16S rRNA gene sequences by distance matrix and parsimony methods indicated that six strains of bacteria isolated from deep saturated Atlantic coastal plain sediments were closely related to the genus Spltingomonas. Five of the strains clustered with, but were distinct from, Sphingomonas capsulata, whereas the sixth strain was most closely related to Blastobacter natatorius. The five strains that clustered with S. capsulata, all of which could degrade aromatic compounds, were gram-negative, non-spore-forming, nonmotile, rod shaped organisms that produced small, yellow colonies on complex media. Their G + C contents ranged from 60.0 to 65.4 mol%, and the predominant isoprenoid quinone was ubiquinone Q-10. All of the strains were aerobic and catalase positive. Indole, urease, and arginine dihydrolase were not produced. Gelatin was not liquified, and glucose was not fermented. Sphingolipids were present in all strains; 2OH14:0 was the major hydroxy fatty acid, and 18:1 was a major constituent of cellular lipids. Acid was produced oxidatively from pentoses, hexoses, and disaccharides, but not from polyalcohols and indole. All of these characteristics indicate that the five aromatic-degrading strains should be placed in the genus Sphingomonas as currently defined. Phylogenetic analysis of 16S rRNA gene sequences, DNA-DNA reassociation values, BOX-PCR genomic fingerprinting, differences in cellular lipid composition, and differences in physiological traits all indicated that the five strains represent three previously undescribed Sphingomonas species. Therefore, we propose the following new species: Sphingomonas ar aromaticivorans (type strain, SMCC P199), Sphingomonas subterranea (type strain, SMCC B0478), and Sphingomonas stygia (type strain, SMCC B0712).