Phenotypic detection of mecC-MRSA: cefoxitin is more reliable than oxacillin
Phenotypic detection of mecC-MRSA: cefoxitin is more reliable than oxacillin
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DOI：
10.1093/jac/dkt341
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发表时间：
2014-01-01
影响因子：
5.2
通讯作者：
Hill, Robert
中科院分区：
文献类型：
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作者：
Skov, Robert;Larsen, Anders Rhod;Hill, Robert
目的：目的探讨头孢西丁和苯唑西林检测mecC阳性金黄色葡萄球菌的可靠性。采用微量肉汤稀释法、琼脂稀释法、Etest法和纸片扩散法在不同类型的培养基上对金黄色葡萄球菌分离株进行了研究。这些数据进行了解释的实用性头孢西丁和苯唑西林结合所述的方法检测mecC阳性的isolates.Results：头孢西丁与Mueller-Hinton媒体从Becton Dickinson和Oxoid检测所有mecC阳性的菌株时，肉汤微量稀释，琼脂稀释和光盘扩散测试。通过Etest，1个分离株为假敏感。bioMerieux的Mueller-Hinton琼脂检测这些分离株的能力要低得多。当使用Iso-Sensitest和哥伦比亚琼脂时，一个分离株通过琼脂稀释法假敏感。在Iso-Sensitest琼脂上使用头孢西丁进行纸片扩散，漏掉了29%的分离株。结论：头孢西丁与苯唑西林联合应用EUCAST方法能检出所有mecC阳性菌株，苯唑西林与哥伦比亚琼脂+2%NaCl琼脂稀释法能检出所有mecC阳性菌株。与BSAC推荐的Iso-Sensitest相比，这些方法及其伴随的琼脂应是首选。应注意的是，对于纸片扩散，来自bioMerieux的Mueller-Hinton培养基表现不佳，26%-47%的mecC分离株为假敏感。
Objectives: To investigate the reliability of cefoxitin and oxacillin for the detection of mecC-positive Staphylococcus aureus.Methods: The susceptibility to cefoxitin and oxacillin of 62 mecC-positive S. aureus isolates was investigated using broth microdilution, agar dilution, Etest and disc diffusion on different types of media. The data were interpreted for the utility of cefoxitin and oxacillin in conjunction with the stated methodologies for the detection of mecC-positive isolates.Results: Cefoxitin with Mueller-Hinton media from Becton Dickinson and Oxoid detected all mecC-positive isolates when tested by broth microdilution, agar dilution and disc diffusion. By Etest, one isolate was falsely susceptible. Mueller-Hinton agar from bioMerieux was substantially less able to detect these isolates. One isolate was falsely susceptible by agar dilution when using Iso-Sensitest and Columbia agar. Disc diffusion using cefoxitin on Iso-Sensitest agar missed 29% of the isolates. For oxacillin, only agar dilution on Columbia agar + 2% NaCl was able to detect all mecC-positive isolates successfully.Conclusions: Cefoxitin used with EUCAST methodology and oxacillin used with agar dilution on Columbia agar + 2% NaCl detected all mecC-positive isolates. These methods with their concomitant agars should be preferred over Iso-Sensitest, which is recommended by the BSAC. It should be noted that for disc diffusion Mueller-Hinton media from bioMerieux performed poorly, with 26%-47% of mecC isolates being falsely susceptible.