Identification of a new pathogen-induced member of the subtilisin-like processing protease family from plants
Identification of a new pathogen-induced member of the subtilisin-like processing protease family from plants
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DOI：
10.1074/jbc.272.22.14412
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发表时间：
1997-05-30
影响因子：
4.8
通讯作者：
Vera, P
中科院分区：
文献类型：
--
作者：
Tornero, P;Conejero, V;Vera, P
利用生物化学、免疫学和分子生物学的方法，我们鉴定并克隆了一个编码番茄蛋白酶的cDNA（Lycopersicon esculentum）植物（P69 B），其是由于感染柑橘裂皮类病毒而在植物中激活的蛋白水解系统的一部分，这种新的蛋白酶在氨基酸序列和结构组织方面密切相关，与先前鉴定的致病相关的枯草杆菌蛋白酶样蛋白酶（Tornero，P，Conejero，V，和Vera，P，（1996）Proc，Natl，Acad，Sci，U.S.A.93，6332 - 6337），P69 B基因全长745个氨基酸，起始于一个可裂解的信号肽，含有一个前结构域和一个631个氨基酸的成熟结构域，该成熟结构域与细菌枯草杆菌蛋白酶和真核生物类Kex 2蛋白酶的催化模块同源。和Ser残基，它们与该蛋白酶家族的催化三联体一致，在P69 B中是保守的，北方印迹和逆转录酶-聚合酶链反应分析表明，作为类病毒感染的结果，我们建议，P69 B是一个复杂的基因家族的植物Kex 2/枯草杆菌蛋白酶样蛋白酶的成员，推测参与了一些特定的蛋白水解事件在植物发病过程中激活，并发生在细胞外基质。
By using biochemical, immunological, and molecular strategies we have identified and cloned a cDNA encoding a protease from tomato (Lycopersicon esculentum) plants (P69B) that is part of a proteolytic system activated in the plant as a result of infection with citrus exocortis viroid, This new protease is closely related, in terms of amino acid sequence and structural organization, to the previously identified pathogenesis-related subtilisin-like protease (Tornero, P,, Conejero, V,, and Vera, P, (1996) Proc, Natl, Acad, Sci, U.S.A. 93, 6332 - 6337), The 745-residue amino acid sequence of P69B begins with a cleavable signal peptide, contains a prodomain and a 631-residue mature domain which is homologous 60 the catalytic modules of bacterial subtilisins and eukaryotic Kex2-like proteases, Within the catalytic domain, the essential Asp, His, and Ser residues that conform the catalytic triad of this family of proteases are conserved in P69B, Northern blot and reverse transcriptase-polymerase chain reaction analysis demonstrated widespread induced expression of the 2.5-kilobase hybridizing mRNA in plant tissues as a consequence of viroid infection, We propose that P69B is a member of a complex gene family of plant Kex2/subtilisin-like proteases presumably involved in a number of specific proteolytic events activated during pathogenesis in plants and that takes place in the extracellular matrix.