The Periosteum as a Cellular Source for Functional Tissue Engineering
The Periosteum as a Cellular Source for Functional Tissue Engineering
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DOI：
10.1089/ten.tea.2008.0244
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发表时间：
2009-09-01
影响因子：
4.1
通讯作者：
Jacobs, Christopher R.
中科院分区：
文献类型：
--
作者：
Arnsdorf, Emily J.;Jones, Luis M.;Jacobs, Christopher R.
骨膜是一种由成纤维细胞、成骨细胞和祖细胞组成的特殊纤维组织，基于其可及性、骨膜细胞在体内和体外快速增殖的能力以及观察到的这些细胞的分化潜力，可能是组织工程的最佳细胞来源。然而，骨膜来源的细胞作为组织工程来源的功能性用途需要了解此类细胞精心设计不同组织基质的能力。在这项研究中，我们将一群贴壁的原代骨膜衍生细胞置于成脂和成骨培养条件下。骨膜细胞的定型倾向与明确表型纯的 NIH3T3 成纤维细胞和 MC3T3-E1 成骨细胞系群体的定型倾向形成鲜明对比。我们的结果表明，骨膜细胞和 NIH3T3 成纤维细胞的异质群体能够表达具有相似潜力的成骨细胞样和脂肪细胞样标记物。这就提出了一个问题：骨膜内的成纤维细胞实际上是否可能具有像祖细胞一样的行为并在组织的多谱系潜力中发挥作用，或者骨膜内是否存在真正的干细胞。此外，这项研究表明，扩展的骨膜培养物可能是组织工程应用的来源，而无需通过分子标记进行广泛的富集或分类。因此，这项研究为未来的研究奠定了基础，这些研究将更深入地列举控制骨膜细胞分化的细胞来源和分子事件。
The periosteum, a specialized fibrous tissue composed of fibroblast, osteoblast, and progenitor cells, may be an optimal cell source for tissue engineering based on its accessibility, the ability of periosteal cells to proliferate rapidly both in vivo and in vitro, and the observed differentiation potential of these cells. However, the functional use of periosteum-derived cells as a source for tissue engineering requires an understanding of the ability of such cells to elaborate matrix of different tissues. In this study, we subjected a population of adherent primary periosteum-derived cells to both adipogenic and osteogenic culture conditions. The commitment propensity of periosteal cells was contrasted with that of well-characterized phenotypically pure populations of NIH3T3 fibroblast and MC3T3-E1 osteoblast cell lines. Our results demonstrate that the heterogeneous populations of periosteal cells and NIH3T3 fibroblasts have the ability to express both osteoblast-like and adipocyte-like markers with similar potential. This raises the question of whether fibroblasts within the periosteum may, in fact, have the potential to behave like progenitor cells and play a role in the tissue's multilineage potential or whether there are true stem cells within the periosteum. Further, this study suggests that expanded periosteal cultures may be a source for tissue engineering applications without extensive enrichment or sorting by molecular markers. Thus, this study lays the groundwork for future investigations that will more deeply enumerate the cellular sources and molecular events governing periosteal cell differentiation.