The inducible antibacterial peptides of the hemipteran insect Palomena prasina: Identification of a unique family of proline-rich peptides and of a novel insect defensin
The inducible antibacterial peptides of the hemipteran insect Palomena prasina: Identification of a unique family of proline-rich peptides and of a novel insect defensin
复制标题
DOI：
10.1016/0022-1910(95)00085-2
复制
发表时间：
1996-01-01
影响因子：
2.2
通讯作者：
Bulet, P
中科院分区：
文献类型：
--
作者：
Chernysh, S;Cociancich, S;Bulet, P
在细菌攻击诱导下，Prasina成虫无细胞血淋巴中出现较强的抗菌活性，我们分离了几种能够引起这种活性的多肽，并通过Edman降解和质谱分析确定了它们的一级序列。五个分离的多肽中有四个是密切相关的小分子、强阳离子、富含脯氨酸的多肽。对于这些新的多肽，我们建议将其命名为Metalnikowins，我们化学合成了Metalnikowin I，并分析了其活性谱，主要针对革兰氏阴性菌，Metalnikowin似乎具有抑菌作用。我们从棕榈科植物中分离到的第五个诱导肽是一种43个残基的昆虫防御素的新亚型。
Bacterial challenge induced the appearance of a strong antibacterial activity in the cell-free hemolymph of adult Palomena prasina (Hemiptera), We isolated several peptides that were capable of causing this activity and determined their primary sequence by Edman degradation and mass spectrometry. Four of the five isolated peptides are closely related small, strongly cationic, proline-rich peptides. They are not posttranslationnally modified, We propose the name of metalnikowins for these novel peptides, We have chemically synthesized metalnikowin I and analyzed its activity spectrum, which is primarily directed against Gram negative bacteria, Metalnikowin appears to be bacteriostatic. The fifth of the induced peptides which we have isolated from Palomena is a 43-residue novel isoform of insect defensins.