Filtered backprojection for the reconstruction of a high-resolution (4,2)D CH3-NHNOESY spectrum on a 29 kDa protein
Filtered backprojection for the reconstruction of a high-resolution (4,2)D CH3-NHNOESY spectrum on a 29 kDa protein
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DOI：
10.1021/ja053110k
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发表时间：
2005-08-24
影响因子：
15
通讯作者：
Zhou, P
中科院分区：
文献类型：
--
作者：
Coggins, BE;Venters, RA;Zhou, P
投影−重建（PR）核磁共振能够快速收集多维核磁共振数据。NOESY对现有的重建算法来说是一个特别困难的挑战，因为它需要在全灵敏度下对未知数量的峰值进行定量构建。我们已经证明了PR - NMR在NOESY上的成功应用，通过从2D投影中确定29 kDa蛋白（人碳酸酐酶II）的4D甲基/酰胺NOESY光谱，使用过滤后的反向投影进行重建。与三维控制谱相比，所有预期的峰都被忠实地重建，具有正确的体积，没有伪影。因此，滤波后的反投影提供了一种方法，在常规3D数据收集所需的时间内获得高分辨率的4D NOESY数据。
Projection−reconstruction (PR) NMR enables rapid collection of multidimensional NMR data. NOESY represents a particularly difficult challenge for currently existing reconstruction algorithms, as it requires thequantitativereconstruction of an unknown number of peaks, at full sensitivity. We have demonstrated the successful application of PR−NMR to NOESY by determining the 4D methyl/amide NOESY spectrum of a 29 kDa protein, human carbonic anhydrase II, from 2D projections, using filtered backprojection for reconstruction. Compared with a 3D control spectrum, all expected peaks were faithfully reconstructed, with correct volumes and with no artifacts. Filtered backprojection thus provides a way to obtain high-resolution 4D NOESY data in the time required for conventional 3D data collection.