Staphylococcus aureus SrrAB Affects Susceptibility to Hydrogen Peroxide and Co-Existence with Streptococcus sanguinis.
Staphylococcus aureus SrrAB Affects Susceptibility to Hydrogen Peroxide and Co-Existence with Streptococcus sanguinis.
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DOI：
10.1371/journal.pone.0159768
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Komatsuzawa H
中科院分区：
文献类型：
--
作者：
Oogai Y;Kawada-Matsuo M;Komatsuzawa H
金黄色葡萄球菌是一种病原体，也是一种在人体内发现的细菌。细菌双组分系统（TCS）感知并响应环境压力，其中包括由其他细菌产生的抗菌剂。在这项研究中，我们分析了TCS SrrAB和对过氧化氢（H2O2）的敏感性之间的关系，过氧化氢是由血链球菌，这是一种口腔链球菌。srrA失活的S.金黄色葡萄球菌突变株对S.吸血鬼我们研究了抗氧化因子在突变体中的表达。突变体中katA的表达显着高于野生型（WT）在存在或不存在0.4 mM H2O2。突变体中dps的表达在H2O2存在下比WT显著增加，但在H2O2不存在下没有。与WT相比，katA或dps失活的突变体对H2O2具有高易感性。此外，我们发现，一氧化氮解毒蛋白（flavohemoglobin：Hmp），这是由SrrAB调节，与H2O2敏感性。hmp失活突变体对S.血比WT。当srrA失活突变体或野生型与S.突变体的群体比例显著高于野生型。总之，SrrAB调节katA，dps和hmp的表达，并影响H2O2敏感性。我们的研究结果表明，SrrAB在体内与S.金黄色葡萄球菌（S. aureus）吸血鬼
Staphylococcus aureus is a pathogen and a commensal bacterial species that is found in humans. Bacterial two-component systems (TCSs) sense and respond to environmental stresses, which include antimicrobial agents produced by other bacteria. In this study, we analyzed the relation between the TCS SrrAB and susceptibility to the hydrogen peroxide (H2O2) that is produced by Streptococcus sanguinis, which is a commensal oral streptococcus. An srrA-inactivated S. aureus mutant demonstrated low susceptibility to the H2O2 produced by S. sanguinis. We investigated the expression of anti-oxidant factors in the mutant. The expression of katA in the mutant was significantly higher than in the wild-type (WT) in the presence or absence of 0.4 mM H2O2. The expression of dps in the mutant was significantly increased compared with the WT in the presence of H2O2 but not in the absence of H2O2. A katA or a dps-inactivated mutant had high susceptibility to H2O2 compared with WT. In addition, we found that the nitric oxide detoxification protein (flavohemoglobin: Hmp), which is regulated by SrrAB, was related to H2O2 susceptibility. The hmp-inactivated mutant had slightly lower susceptibility to the H2O2 produced by S. sanguinis than did WT. When a srrA-inactivated mutant or the WT were co-cultured with S. sanguinis, the population percentage of the mutant was significantly higher than the WT. In conclusion, SrrAB regulates katA, dps and hmp expression and affects H2O2 susceptibility. Our findings suggest that SrrAB is related in vivo to the co-existence of S. aureus with S. sanguinis.