5 SWI GENES ARE REQUIRED FOR EXPRESSION OF THE HO GENE IN YEAST
5 SWI GENES ARE REQUIRED FOR EXPRESSION OF THE HO GENE IN YEAST
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DOI：
10.1016/0022-2836(84)90315-2
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发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
HERSKOWITZ, I
中科院分区：
文献类型：
--
作者：
STERN, M;JENSEN, R;HERSKOWITZ, I
酵母中的高频率交配型相互转换需要HO基因，其编码启动转换过程的位点特异性核酸内切酶。分离并分析开关缺陷突变体。这些突变体定义了5个互补和连接基团，SWI 1至SWI 5。两种测定，北方杂交和β-在含有HO-lacZ融合的菌株中的半乳糖苷酶活性，表明缺陷突变体SWI基因不能表达HO基因。所有的swi突变体都表现出其他表型，最值得注意的是在SWI 4和SWI 1、SWI 2或SWI 3中缺陷的双突变体的不存活性。SWI基因显然以某种方式作为HO表达的正调节因子发挥作用，并具有额外的细胞作用。
High-frequency mating type interconversion in yeast requires the HO gene, which encodes a site-specific endonuclease that initiates the switching process. Switching-defective mutants were isolated and analyzed. These mutants define 5 complementation and linkage groups, SWI 1 to SWI 5. Two assays, Northern hybridization and .beta.-galactosidase activity in strains containing an HO-lacZ fusion, showed that defective mutant SWI gene failed to express the HO gene. All of the swi mutants exhibit other phenotypes, the most notable being the inviability of double mutants defective in SWI 4 and in either SWI 1, SWI 2 or SWI 3. The SWI genes apparently function in some way as positive regulators of HO expression and have additional cellular roles.