Open-field behavior of house mice selectively bred for high voluntary wheel-running
Open-field behavior of house mice selectively bred for high voluntary wheel-running
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DOI：
10.1023/a:1012283426530
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发表时间：
2001-05-01
影响因子：
2.6
通讯作者：
Garland, T
中科院分区：
文献类型：
--
作者：
Bronikowski, AM;Carter, PA;Garland, T
开放式行为测试通常用于测试啮齿动物的活动能力和情绪性。我们对家鼠(Mus Home Ticus)进行了旷场试验，这些家鼠来自四个重复品系和四个重复的随机繁殖对照品系。连续2天在1m(2)的露天场地用摄像机记录小鼠3min。在试验期间，来自选定品系的小鼠与对照组小鼠在行走距离、排便、在室内停留的时间或距离竞技场中心的平均距离方面没有统计上的差异。因此，我们发现几乎没有证据表明，传统定义的开阔场地行为与车轮奔跑行为存在遗传关联。这一结果是对经典研究的有用的反向测试，经典研究报告称，在被选为增加野外活动的小鼠中，轮子跑得没有增加。然而，来自选定品系的小鼠比对照品系的小鼠在旅行路线上转向更少，来自选定品系的雌性小鼠到达墙的旅行时间更慢(潜伏期更长)。我们在历史上的开阔场地测试和新定义的开阔场地活动测量的背景下讨论了这些结果。
Open-field behavioral assays are commonly used to test both locomotor activity and emotionality in rodents. We performed open-field tests on house mice (Mus domesticus) from four replicate lines genetically selected for high voluntary wheel-running for 22 generations and from four replicate random-bred control lines. Individual mice were recorded by video camera for 3 min in a 1-m(2) open-field arena on 2 consecutive days. Mice from selected lines showed no statistical differences from control mice with respect to distance traveled, defecation, time spent in the interior, or average distance from the center of the arena during the trial. Thus, we found little evidence that open-field behavior, as traditionally defined, is genetically correlated with wheel-running behavior. This result is a useful converse test of classical studies that report no increased wheel-running in mice selected for increased open-field activity. However, mice from selected lines turned less in their travel paths than did control-line mice, and females from selected lines had slower travel times (longer latencies) to reach the wall. We discuss these results in the context of the historical open-field test and newly defined measures of open-field activity.