Differences in Protein Expression between the U251 and U87 Cell Lines
Differences in Protein Expression between the U251 and U87 Cell Lines
复制标题
U251 和 U87 细胞系之间蛋白质表达的差异。
DOI：
10.5137/1019-5149.jtn.17746-16.1
复制
发表时间：
2017-01-01
影响因子：
0.8
通讯作者：
Qi, Songtao
中科院分区：
文献类型：
--
作者：
Li, Hezhen;Lei, Bingxi;Qi, Songtao
目标
U251和U87细胞系通常被用作胶质母细胞瘤的实验模型。然而，这些细胞在增殖、侵袭和迁移方面表现出显著的差异。本研究的目的是比较U251和U87细胞系的蛋白质表达谱，以便为观察到的表型差异提供分子基础。
材料和方法
相对和绝对定量等压标签(ITRAQ)和基因本体论(GO)分析分别用于检测差异表达的蛋白质和预测蛋白质功能。
结果
与U87细胞相比，U251细胞中有244个蛋白质高表达，而263个蛋白质表达水平较低。特别是定量聚合酶链式反应证实，U251细胞株与U87细胞株相比，C10orf58、FLNC、PDLIM1、TPM4蛋白表达水平较高，而MYH10、PSIP1、SYNM、SLC9A3R2、BCAM蛋白表达水平较低。当对iTRAQ结果进行GO分析时，发现在U251细胞中高表达的蛋白质在分子功能、生物学过程、细胞分布和与其相关的细胞途径方面与在U87细胞中检测到的高表达蛋白质不同。
结论
U251和U87细胞株之间差异表达的蛋白质与烟酰胺核苷酸代谢、RNA剪接、糖酵解和嘌呤代谢途径的调节有关。对这些通路的进一步研究可能确定这些不同的通路是否可以解释观察到的U87和U251 GBM细胞系之间的表型差异。
AIM
The U251 and U87 cell lines are commonly used as experimental models of glioblastoma. However, these cells exhibit significant differences in their proliferation, invasion, and migration. The aim of the present study was to compare the protein expression profiles of the U251 and U87 cell lines in order to provide a molecular basis for the observed phenotypic differences.
MATERIAL AND METHODS
Isobaric tags for relative and absolute quantitation (iTRAQ) and gene ontology (GO) analyses were performed to detect differentially expressed proteins and to predict protein functions, respectively.
RESULTS
Two hundred and forty-four proteins were highly expressed, while 263 proteins exhibited lower levels of expression, in the U251 cells compared to the U87 cells. In particular, higher expression levels of the proteins, C10orf58, FLNC, PDLIM1, TPM4, and lower expression levels of MYH10, PSIP1, SYNM, SLC9A3R2, BCAM, were verified by qPCR in the U251 cell line versus the U87 cell line. When a GO analysis was applied to the iTRAQ results, the proteins that were highly expressed in the U251 cells were found to differ in their molecular functions, biological processes, cellular distribution, and cellular pathways associated with them compared with the highly expressed proteins detected in the U87 cells.
CONCLUSION
Differentially expressed proteins between the U251 and U87 cell lines are associated with regulation of nicotinamide nucleotide metabolism, RNA splicing, glycolysis, and purine metabolism pathways. Further studies on these pathways may identify whether these various pathways account for the observed phenotype differences between the U87 and U251 GBM cell lines.