A molecular fluorescent dye for specific staining and imaging of RNA in live cells: a novel ligand integration from classical thiazole orange and styryl compounds
A molecular fluorescent dye for specific staining and imaging of RNA in live cells: a novel ligand integration from classical thiazole orange and styryl compounds
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DOI：
10.1039/c5cc05551b
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Chow, Cheuk-Fai
中科院分区：
文献类型：
--
作者：
Lu, Yu-Jing;Deng, Qiang;Chow, Cheuk-Fai
一种新的RNA选择性荧光染料与噻唑橙子和p-（甲硫基）苯乙烯基部分集成显示更好的核仁RNA染色和成像性能在活细胞中比商业染料。它还表现出优异的光稳定性、细胞耐受性和与4 '，6-二脒基-2-苯基吲哚的复染相容性，用于生物测定中的特异性RNA-DNA共定位。
A new RNA-selective fluorescent dye integrated with a thiazole orange and a p-(methylthio) styryl moiety shows better nucleolus RNA staining and imaging performance in live cells than the commercial stains. It also exhibits excellent photostability, cell tolerance, and counterstain compatibility with 4',6-diamidino-2-phenylindole for specific RNA-DNA colocalization in bioassays.