INTERCELLULAR COMMUNICATION BETWEEN RAT ANTERIOR-PITUITARY CELLS
INTERCELLULAR COMMUNICATION BETWEEN RAT ANTERIOR-PITUITARY CELLS
复制标题
DOI：
10.1002/ar.1092240410
复制
发表时间：
1989-08-01
影响因子：
--
通讯作者：
HERBERT, DC
中科院分区：
文献类型：
--
作者：
SOJI, T;HERBERT, DC
采用免疫组织化学、扫描电镜、冷冻断裂电镜和常规透射电镜等方法研究了60日龄雄性大鼠垂体前叶内细胞间的通讯。一个致密的细胞网状网络的滤泡星状细胞的胞质突起被发现含有免疫反应S-100蛋白，并观察到整个垂体前叶。非免疫反应细胞，这是颗粒状的，位于每个网络的中心。几乎所有的颗粒细胞都位于滤泡星状细胞附近。扫描电子显微镜显示，腺体由微小叶包围的基膜。小叶表面有血管，血管分支侵入小叶内部。细胞质突起形成突出于小叶外的滤泡星状细胞。冷冻断裂电子显微镜显示质膜的P面上存在许多膜内颗粒。在细胞表面上散布的是形成间隙连接的颗粒群。在微绒毛碎片簇附近也观察到代表紧密连接的脊状网状结构。形成小间隙连接的颗粒簇位于紧密连接的网络之间。小缝隙连接清楚地观察到传统的电子显微镜之间的交界复合物的冷冻断裂电子显微镜观察到的方式类似。细长的滤泡星状细胞的细胞质过程接触附近的基底层，并毗邻小缝隙连接。含有缝隙连接的非颗粒细胞与不存在缝隙连接的非颗粒细胞的比例约为1：1。差距结的尺寸范围为50 nm至3 μ m。沿颗粒细胞的质膜未观察到沿着的缝隙连接。大鼠垂体前叶内细胞间通讯系统的意义在于在一个缺乏直接神经支配的器官中建立一种快速传递信息的机制。
Cell-to-cell communication within the rat anterior pituitary was investigated in 60-day-old male rats with immunohistochemistry, scanning electron microscopy, freeze-fracture electron microscopy, and conventional transmission electron microscopy. A dense cytoreticular network of cytoplasmic processes from the folliculostellate cells was found to contain immunoreactive S-100 protein and was observed throughout the anterior pituitary. Nonimmunoreactive cells, which were granular, were situated in the center of each network. Almost all of the granulated cells were situated in close proximity to the folliculostellate cells. Scanning electron microscopy revealed that the gland consisted of microlobules enclosed by a basal lamina. On the surface of the microlobules were blood vessels whose branches invaded its internal structures. Cytoplasmic processes form folliculostellate cells projected outside the microlobule. Freeze-fracture electron microscopy demonstrated the presence of numerous intramembranous particles on the P-face of the plasma membrane. Scattered on the cell surface were groups of particles forming gap junctions. Meshworks of ridges which were representations of tight junctions were also observed near clusters of microvillous fragments. Clusters of particles forming small gap junctions were located between the meshworks of tight junctions. Small gap junctions were clearly observed by conventional electron microscopy between junctional complexes in a manner similar to that seen by freeze-fracture electron microscopy. Slender cytoplasmic processes of folliculostellate cells came in contact near the basal lamina and were adjoined by small gap junctions. The ratio of nongranular cells which contained gap junctions to those in which the junctions were absent was about 1:1. The size of the gap junctions ranged from 50 nm to 3 .mu.m. No gap junctions were observed along the plasma membranes of the granular cells. The significance of an intercellular communication system within the anterior pituitary gland of the rat is to establish a mechanism for rapid transmission of information in an organ which lacks direct innervation.