Effects of oleamide on choline acetyltransferase and cognitive activities
Effects of oleamide on choline acetyltransferase and cognitive activities
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DOI：
10.1271/bbb.67.1284
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发表时间：
2003-06-01
影响因子：
1.6
通讯作者：
Shin, DH
中科院分区：
文献类型：
--
作者：
Heo, HJ;Park, YJ;Shin, DH
我们筛选了50种韩国传统的天然植物，以测量对胆碱乙酰转移酶的激活作用和东莨菪碱诱导的健忘症的衰减。在50种供试植物中，枣的甲醇提取物对胆碱乙酰转移酶的体外激活作用最强（34.1%）。通过对酸枣仁进行分级分离，最终确定其有效成分为顺式-9-十八烯酰胺（油酰胺）。分离后，油酰胺显示出65%的活化效果。在被动回避试验和Y-迷宫试验中，给予小鼠油酰胺（0.32%）可显著逆转东莨菪碱诱导的记忆和/或认知障碍。给小鼠注射东莨菪碱后，其被动回避试验（避暗潜伏期减少31%）和Y-迷宫试验（交替行为减少16%）的表现受损。相比之下，在东莨菪碱注射前用油酰胺处理的小鼠免受这些变化的影响（跨步潜伏期减少12-25%;交替行为减少1-10%）。这些结果表明，油酰胺应该是一个有用的化学预防剂对阿尔茨海默氏病。
We screened 50 Korean traditional natural plants to measure the activation effect on choline acetyltransferase and attenuation of scopolamine-induced amnesia. The methanolic extracts from Zizyphus jujuba among the tested 50 plants, showed the highest activatory effect (34.1%) on choline acetyltransferase in vitro. By sequential fractionation of Zizyphus jujuba, the active component was finally identified as cis-9-octadecenoamide (oleamide). After isolation, oleamide showed a 65% activation effect. Administration of oleamide (0.32%) to mice significantly reversed the scopolamine-induced memory and/or cognitive impairment in the passive avoidance test and Y-maze test. Injection of scopolamine to mice impaired performance on the passive avoidance test (31% decrease in step-through latency), and on the Y-maze test (16% decrease in alternation behavior). In contrast, mice treated with oleamide before scopolamine injection were protected from these changes (12-25% decrease in step-through latency; 1-10% decrease in alternation behavior). These results suggest that oleamide should be a useful chemo-preventive agent against Alzheimer's disease.