Teaching an Old Shell New Tricks: Extracting DNA from Current, Historical, and Ancient Mollusk Shells
Teaching an Old Shell New Tricks: Extracting DNA from Current, Historical, and Ancient Mollusk Shells
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DOI：
10.1093/biosci/biaa164
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发表时间：
2021-01-20
期刊：
影响因子：
10.1
通讯作者：
Parent，Christine E.
中科院分区：
文献类型：
--
作者：
Martin，Kelly R.;Waits，Lisette P.;Parent，Christine E.
非常规DNA来源的使用已经增加，因为传统样本的获取可能是侵入性的、破坏性的或不可能的。软体动物是一个新的遗传来源是至关重要的群体，但方法仍然相对不发达。许多物种在生态上和水产养殖生产中具有重要意义。然而，软体动物在所有分类群中的灭绝数量最多。传统上，软体动物壳材料用于形态学研究，直到最近才用于DNA研究。在本文中，我们回顾了壳DNA提取的研究，发现有效的程序考虑分类特异性的生物学特性，环境条件，实验室方法和研究目的。重要的是，不能孤立地考虑这些因素，因为它们在贝壳DNA的长期保存和恢复中具有基本的、有时是相互的关系和影响。贝壳DNA的成功回收可以促进对紧迫的生态和进化问题的研究，并为保护软体动物多样性的保护策略提供信息。
The use of unconventional DNA sources has increased because the acquisition of traditional samples can be invasive, destructive, or impossible. Mollusks are one group for which novel genetic sources are crucial, but methodology remains relatively undeveloped. Many species are important ecologically and in aquaculture production. However, mollusks have the highest number of extinctions of any taxonomic group. Traditionally, mollusk shell material was used for morphological research and only recently has been used in DNA studies. In the present article, we review the studies in which shell DNA was extracted and found that effective procedures consider taxon-specific biological characteristics, environmental conditions, laboratory methods, and the study objectives. Importantly, these factors cannot be considered in isolation because of their fundamental, sometimes reciprocal, relationships and influence in the long-term preservation and recovery of shell DNA. Successful recovery of shell DNA can facilitate research on pressing ecological and evolutionary questions and inform conservation strategies to protect molluscan diversity.