Hantaviruses in Finnish soricomorphs: Evidence for two distinct hantaviruses carried by Sorex araneus suggesting ancient host-switch
Hantaviruses in Finnish soricomorphs: Evidence for two distinct hantaviruses carried by Sorex araneus suggesting ancient host-switch
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DOI：
10.1016/j.meegid.2014.06.023
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发表时间：
2014-10-01
影响因子：
3.2
通讯作者：
Vapalahti, Olli
中科院分区：
文献类型：
--
作者：
Ling, Jiaxin;Sironen, Tarja;Vapalahti, Olli
汉坦病毒是由啮齿类动物、鼩鼱和鼹鼠以及蝙蝠携带的新出现的病毒。在芬兰，普马拉病毒（PUUV）多年来一直是唯一检测到的汉坦病毒。然而，2009 年，从 1982 年在芬兰采集的普通鼩鼱 (Sorex araneus) 档案样本中发现了 Seewis 病毒 (SWSV)。为了阐明芬兰体型中汉坦病毒的多样性，对 180 个个体进行了筛选，代表 2001 年至 2012 年捕获的 7 个物种：使用 RT-PCR 筛选汉坦病毒 RNA，并使用针对截短 SWSV 核衣壳蛋白的多克隆抗体进行免疫印迹筛选汉坦病毒抗原。汉坦病毒RNA总体流行率为14%（26/180），20只SWSV RT-PCR阳性鼩鼱中有9只可检测到抗原。遗传分析显示，芬兰有四种由soricomorph传播的汉坦病毒，包括水鼩（Neomys fodiens）中的Boginia病毒（BOGV）和侏儒鼩（Sorex minutus）中的阿西卡拉病毒（ASIV）。有趣的是，在两个研究地点，普通鼩鼱携带两种不同的汉坦病毒株：Seewis病毒和一种新的独特的、遗传上遥远的（氨基酸水平同一性为57%）病毒（阿尔泰样病毒），该病毒与汉坦病毒基础系统群I中的病毒聚集在一起，在氨基酸水平上具有62-67%的同一性。这是在一个宿主物种群体中同时传播的两种明显不同的汉坦病毒物种共存的第一个证据。这些发现表明，存在着从未知宿主到蜘蛛链球菌的古老宿主转换事件。此外，对部分S和M片段序列的系统发育分析表明，芬兰的SWSV代表了欧洲独特的基因型。 (C) 2014 Elsevier B.V. 保留所有权利。
Hantaviruses are emerging viruses carried by rodents, soricomorphs (shrews and moles) and bats. In Finland, Puumala virus (PUUV) was for years the only hantavirus detected. In 2009, however, Seewis virus (SWSV) was reported from archival common shrew (Sorex araneus) samples collected in 1982 in Finland. To elucidate the diversity of hantaviruses in soricomorphs in Finland, 180 individuals were screened, representing seven species captured from 2001 to 2012: hantavirus RNA was screened using RT-PCR, and hantaviral antigen using immunoblotting with polyclonal antibodies raised against truncated SWSV nucleocapsid protein. The overall hantavirus RNA prevalence was 14% (26/180), antigen could be demonstrated in 9 of 20 SWSV RT-PCR positive common shrews. Genetic analyses revealed that four soricomorph-borne hantaviruses circulate in Finland, including Boginia virus (BOGV) in water shrew (Neomys fodiens) and Asikkala virus (ASIV) in pygmy shrew (Sorex minutus). Interestingly, on two study sites, common shrews harbored strains of two different hantaviruses: Seewis virus and a new distinct, genetically distant (identity 57% at amino acid level) virus (Altai-like virus) which clusters together with viruses in the basal phylogroup I of hantaviruses with 62-67% identity at amino acid level. This is the first evidence of coexistence of two clearly distinct hantavirus species circulating simultaneously in one host species population. The findings suggest an ancient host-switching event from a yet unknown host to S. araneus. In addition, phylogenetic analyses of partial S and M segment sequences showed that SWSV in Finland represents a unique genotype in Europe. (C) 2014 Elsevier B.V. All rights reserved.