HIV-infected cells are killed by rCD4-ricin A chain.
HIV-infected cells are killed by rCD4-ricin A chain.
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HIV 感染的细胞被 rCD4-蓖麻毒素 A 链杀死。
DOI：
10.1126/science.2847316
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Vitetta,ES
中科院分区：
文献类型：
--
作者：
Till,MA;Ghetie,V;Gregory,T;Patzer,EJ;Porter,JP;Uhr,JW;Capon,DJ;Vitetta,ES
人类免疫缺陷病毒（HIV）的gp 120包膜糖蛋白在许多HIV感染细胞的表面上表达，与细胞表面分子CD 4结合。以高亲和力结合gp 120的重组CD 4（rCD 4）的可溶性衍生物是用于将细胞毒性试剂靶向HIV感染细胞的有吸引力的载体。可溶性rCD 4与蓖麻毒素的活性亚基结合。该缀合物杀死HIV感染的H9细胞，但对未感染的H9细胞（其不表达gp 120）的毒性为1/1000，并且对Daudi细胞（其表达主要组织相容性II类抗原，细胞表面CD 4的推定天然配体）没有毒性。感染细胞的特异性杀伤可被rgp 120、rCD 4或CD 4上gp 120结合位点的单克隆抗体阻断。
The gp120 envelope glycoprotein of the human immunodeficiency virus (HIV), which is expressed on the surface of many HIV-infected cells, binds to the cell surface molecule CD4. Soluble derivatives of recombinant CD4 (rCD4) that bind gp120 with high affinity are attractive vehicles for targeting a cytotoxic reagent to HIV-infected cells. Soluble rCD4 was conjugated to the active subunit of the toxin ricin. This conjugate killed HIV-infected H9 cells but was 1/1000 as toxic to uninfected H9 cells (which do not express gp120) and was not toxic to Daudi cells (which express major histocompatibility class II antigens, the putative natural ligand for cell surface CD4). Specific killing of infected cells can be blocked by rgp120, rCD4, or a monoclonal antibody to the gp120 binding site on CD4.