Anti-atherogenic effects of the methanol extract of sorbus cortex in atherogenic-diet rats
Anti-atherogenic effects of the methanol extract of sorbus cortex in atherogenic-diet rats
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DOI：
10.1248/bpb.28.1444
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发表时间：
2005-08-01
影响因子：
2
通讯作者：
Lee, HS
中科院分区：
文献类型：
--
作者：
Sohn, EJ;Kang, DG;Lee, HS
本研究旨在探讨花楸皮层甲醇提取物（MSC）是否能通过调节致动脉粥样硬化饮食大鼠血管一氧化氮（NO）和内皮素-1（ET-1）系统来预防动脉粥样硬化的发展。我们的研究结果表明，主动脉NO的生产以及内皮型一氧化氮合酶（ecNOS）的表达显着降低，在致动脉粥样硬化饮食大鼠与对照组相比。在喂食致动脉粥样硬化饮食的大鼠中，主动脉ET-1表达增加，而NF-κ B B p65上调。低剂量（100mg/kg/d）或高剂量（200mg/kg/d）MSC治疗致动脉粥样硬化饮食大鼠不仅导致主动脉NOS/NO系统的显著增加，而且还导致主动脉ET-1表达的降低。低剂量或高剂量MSC慢性治疗也减弱了致动脉粥样硬化饮食大鼠主动脉NF-κ B p65的表达水平。致动脉粥样硬化饮食诱导的粘附分子表达增加，包括细胞间粘附分子-1（ICAM-1），血管细胞粘附分子-1（ICAM-1），和E-选择素与MSC治疗显着下降。从组织病理学检查，MSC治疗显示出减轻在动脉粥样硬化饮食大鼠的主动脉内膜和中膜中注意到的增厚。这些结果表明，MSC影响动脉粥样硬化的过程中，通过抑制促炎症和粘附分子在动脉粥样硬化饮食大鼠，这可能是，至少部分，因果关系与血管活性系统，如NO和ET-1系统的调节。
The present study was designed to examine whether the methanol extract of Sorbus commixta cortex (MSC) could prevent the development of atherosclerosis through regulating the vascular nitric oxide (NO) and endothelin-1 (ET-1) systems in atherogenic-diet rats. Our findings show that aortic NO production as well as endothelial nitric oxide synthase (ecNOS) expression was significantly decreased in atherogenic-diet rats compared with those in the control group. Aortic ET-1 expression was augmented in rats fed an atherogenic-diet while NF-kappa B p65 was upregulated. Treatment of atherogenic-diet rats with either low (100mg/kg/d) or high (200mg/kg/d) doses of MSC led not only to significant increases in the aortic NOS/NO system, but also to decreases in aortic ET-1 expression. The aortic expression level of NF-kappa B p65 was also attenuated in atherogenic-diet rats by chronic treatment with low or high doses of MSC. Atherogenic-diet induced increases in the expression of adhesion molecules including intercellular adhesion molecules-1 (ICAM-1), vascular cell adhesion molecue-1 (ICAM-1), and E-selectin were markedly decreased by treatment with MSC. From the histopathological examination, MSC treatment was shown to lessen the thickening noted in the aortic intima and media of the atherogenic-diet rats. These results suggest that MSC affects the atherogenic process via the suppression of proinflammatory and adhesion molecules in atherogenic-diet rats, which may be, at least in part, causally related with the regulation of vasoactive systems such as the NO and ET-1 systems.