Galanin Inhibits Amylase Secretion from Isolated Rat Pancreatic Acini
Galanin Inhibits Amylase Secretion from Isolated Rat Pancreatic Acini
复制标题
甘丙肽抑制离体大鼠胰腺腺泡的淀粉酶分泌
作者：
B. Ahrén;A. Andrén;H. Nilsson
神经肽甘丙肽存在于胰腺内神经纤维中，已知会影响胰岛激素的分泌。其最有效的作用是抑制胰岛素分泌。在本研究中，我们研究了甘丙肽是否影响离体大鼠胰腺腺泡的淀粉酶分泌。通过胶原酶消化技术分离腺泡，并在存在或不存在甘丙肽的情况下，在添加或不添加胆碱能激动剂卡巴胆碱或胆囊收缩素（CCK-8）的C端八肽的Krebs-Henseleit培养基中孵育45分钟。卡巴胆碱在其最佳浓度M)下，刺激淀粉酶分泌至总淀粉酶含量的11.8±0.5%，而对照组为4.3±2±0.3%（p<0.001）。甘丙肽 (10-8-10-9 M) 将卡巴胆碱诱导的淀粉酶分泌降低至 10.4 ±0.3% (p <0.01)。浓度水平低于 10−7 M 的甘丙肽没有显着影响。在 10−8 M 时，CCK-8 刺激淀粉酶分泌至 9.7 ± 0.6%，而对照组为 5.2 ± 0.3%（p <0.01）。甘丙肽 (10−7 M) 将此刺激降低至 8.0 ± 0.4% (p <0.05)。甘丙肽不影响基础淀粉酶分泌。结论是，胰腺内神经肽甘丙肽弱抑制卡巴胆碱和CCK-8诱导的离体大鼠胰腺腺泡的淀粉酶分泌。因此，甘丙肽不仅能直接影响内分泌，而且能直接影响外分泌胰腺的分泌，尽管其抑制淀粉酶分泌的作用似乎较弱。
The neuropeptide galanin is present in intrapancreatic nerve fibers and is known to affect the secretion of the islet hormones. Its most potent effect is thereby the inhibition of insulin secretion. In the present study, we investigated whether galanin influences amylase secretion from isolated rat pancreatic acini. Acini were isolated by the collagenase digestion technique and incubated for 45 min in a Krebs-Henseleit medium with or without addition of the cholinergic agonist carbachol or the C-terminal octapeptide of cholecystokinin (CCK-8) in the presence or absence of galanin. Carbachol, at its optimal concentration M), stimulated amylase secretion to 11.8 ± 0.5% of total amylase content compared to 4.3 2 0.3% in controls (p <0.001). Galanin, (10-8-10-9 M), reduced the carbachol-induced amylase secretion to 10.4 ±0.3% (p <0.01). Galanin at concentration levels below 10−7 M had no significant effect. At 10−8 M, CCK-8 stimulated amylase secretion to 9.7 ± 0.6% compared with 5.2 ± 0.3% in controls (p <0.01). Galanin (10−7 M) reduced this stimulation to 8.0 ± 0.4% (p <0.05). Galanin did not affect basal amylase secretion. It is concluded that the intrapancreatic neuropeptide galanin weakly inhibits carbachol- and CCK-8-induced amylase secretion from isolated rat pancreatic acini. Thus, galanin has the capability to directly affect not only endocrine but also exocrine pancreatic secretion although its effect of inhibiting amylase secretion seems weak.